Department of Dental Medicine, 1st Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic.
Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
J Oral Pathol Med. 2017 Oct;46(9):834-839. doi: 10.1111/jop.12556. Epub 2017 Feb 23.
Knowledge of the phenotypic pattern of oral squamous epithelium is important in the histopathologic evaluation of lesions including cancer. The literature on normal epithelium is controversial as the phenotype has not been evaluated in samples from completely healthy tissue donors without a history of tobacco and alcohol exposure.
In this study, we evaluated normal upper lip fornix and gingival mucosa from carefully selected young healthy donors without a history of smoking and alcohol exposure, and keratin types 8, 10, 14, and 17, filaggrin, and Ki67 were investigated in these donors. The results were compared with profile of epithelium from leukoplakia.
The results demonstrated that the phenotypic patterns of gingiva and upper lip fornix mucosa were different. Surprisingly, a high proportion of gingival samples exhibited keratin 8 and a suprabasal signal for keratin 14. These patterns were compared with that of human oral leukoplakia, and some phenotypic similarities were noted.
These results demonstrated oral epithelium phenotypic plasticity based on functional requirements of the microenvironment, which can be used in diagnosis.
了解口腔鳞状上皮的表型模式对于包括癌症在内的病变的组织病理学评估很重要。由于文献中关于正常上皮的研究存在争议，因此尚未对无吸烟和饮酒史的完全健康组织供体的样本进行评估。
本研究中，我们评估了来自精心挑选的无吸烟和饮酒史的年轻健康供体的上唇穹窿和牙龈黏膜，研究了这些供体中的角蛋白 8、10、14 和 17、丝聚蛋白和 Ki67。将结果与白斑的上皮特征进行了比较。
结果表明，牙龈和上唇穹窿黏膜的表型模式不同。令人惊讶的是，相当一部分牙龈样本显示角蛋白 8 和角蛋白 14 的上基底层信号。这些模式与人类口腔白斑进行了比较，发现了一些表型上的相似之处。
这些结果表明，口腔上皮具有基于微环境功能需求的表型可塑性，可用于诊断。
