Qiu Tengyang, Teshima Tathyane H N, Hovorakova Maria, Tucker Abigail S
Centre for Craniofacial and Regenerative Biology, Faculty of Dentistry, Oral and Craniofacial Sciences, King's College London, London, United Kingdom.
Institute of Histology and Embryology, First Faculty of Medicine, Charles University in Prague, Prague, Czechia.
Front Physiol. 2020 Jul 16;11:753. doi: 10.3389/fphys.2020.00753. eCollection 2020.
The vestibular lamina (VL) is a transient developmental structure that forms the lip furrow, creating a gap between the lips/cheeks and teeth (oral vestibule). Surprisingly, little is known about the development of the VL and its relationship to the adjacent dental lamina (DL), which forms the teeth. In some congenital disorders, such as Ellis-van Creveld (EVC) syndrome, development of the VL is disrupted and multiple supernumerary frenula form, physically linking the lips and teeth. Here, we assess the normal development of the VL in human embryos from 6.5 (CS19) to 13 weeks of development, showing the close relationship between the VL and DL, from initiation to differentiation. In the anterior lower region, the two structures arise from the same epithelial thickening. The VL then undergoes complex morphogenetic changes during development, forming a branched structure that separates to create the vestibule. Changing expression of keratins highlight the differentiation patterns in the VL, with fissure formation linked to the onset of filaggrin. Apoptosis is involved in removal of the central portion of the VL to create a broad furrow between the future cheek and gum. This research forms an essential base to further explore developmental defects in this part of the oral cavity.
前庭板（VL）是一种短暂的发育结构，它形成唇沟，在嘴唇/脸颊和牙齿之间形成一个间隙（口腔前庭）。令人惊讶的是，关于VL的发育及其与形成牙齿的相邻牙板（DL）的关系知之甚少。在一些先天性疾病中，如埃利斯-范克里弗德（EVC）综合征，VL的发育受到破坏，形成多个额外的系带，将嘴唇和牙齿物理连接起来。在这里，我们评估了人类胚胎从发育6.5周（CS19）到13周时VL的正常发育情况，展示了从起始到分化过程中VL和DL之间的密切关系。在前部下区域，这两个结构起源于相同的上皮增厚。VL在发育过程中经历复杂的形态发生变化，形成一个分支结构，该结构分开以形成前庭。角蛋白表达的变化突出了VL中的分化模式，裂隙形成与丝聚蛋白的出现有关。细胞凋亡参与去除VL的中央部分，以在未来的脸颊和牙龈之间形成一个宽阔的沟。这项研究为进一步探索口腔这一部分的发育缺陷奠定了重要基础。
