Sharif Alireza, Aghakhani Arezoo, Velayati Ali Akbar, Banifazl Mohammad, Sharif Mohammad Reza, Razeghi Effat, Kheirkhah Davood, Kazemimanesh Monireh, Bavand Anahita, Ramezani Amitis
Infectious Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran.
Intervirology. 2016;59(3):179-185. doi: 10.1159/000455124. Epub 2017 Feb 1.
The aim of this study was to evaluate the frequency and genotype of human parvovirus B19 and its relation with anemia among Iranian patients under dialysis.
Fifty hemodialysis (HD) and 33 peritoneal dialysis (PD) patients were enrolled. B19 IgG and IgM antibodies were assessed by ELISA, and the presence of B19 DNA was evaluated by nested PCR. PCR products were sequenced directly and phylogenetic analysis was performed.
In the HD group, the prevalence of B19 antibodies was 54% for IgG and 4% for IgM. B19 DNA was detected in 10% of the cases, and 10% showed B19 IgG and viremia simultaneously. In the PD group, the prevalence of B19 IgG and IgM was 57.6 and 0% respectively, whereas B19 DNA was found in 12.1% of the group. A total of 9.1% showed B19 IgG and viremia concurrently. There was no significant difference regarding anemia and B19 infection in either group. All B19 isolates were clustered in genotype 1A.
Our findings indicate that B19 infection plays no role in leading chronic anemia in dialysis patients. However, persistent B19 viremia and the circulation of the same strains in dialysis patients may indicate a potential risk for the contamination of dialysis equipment and nosocomial spread of B19 infection within dialysis units.
本研究旨在评估伊朗透析患者中人类细小病毒B19的感染频率和基因型及其与贫血的关系。
纳入50例血液透析（HD）患者和33例腹膜透析（PD）患者。采用酶联免疫吸附测定法（ELISA）检测B19 IgG和IgM抗体，采用巢式聚合酶链反应（PCR）评估B19 DNA的存在情况。对PCR产物进行直接测序并进行系统发育分析。
在HD组中，B19 IgG抗体的患病率为54%，IgM抗体的患病率为4%。10%的病例检测到B19 DNA，10%的病例同时出现B19 IgG和病毒血症。在PD组中，B19 IgG和IgM的患病率分别为57.6%和0%，而该组中12.1%的患者检测到B19 DNA。共有9.1%的患者同时出现B19 IgG和病毒血症。两组患者在贫血和B19感染方面均无显著差异。所有B19分离株均聚类于1A基因型。
我们的研究结果表明，B19感染在导致透析患者慢性贫血方面不起作用。然而，透析患者中持续的B19病毒血症以及相同毒株的传播可能表明透析设备污染以及B19感染在透析单元内医院感染传播的潜在风险。
