Schraufnagel D E
Department of Medicine, University of Illinois, Chicago 60680.
Scanning Microsc. 1989 Jun;3(2):575-8.
To determine the importance of the pulmonary or bronchial arteries as the site of injection to cast the lung vasculature rats, we retrospectively compared a group of normal Sprague-Dawley rats that were cast through either the caudal vena cava and right ventricle leading to the pulmonary arteries or through the aorta and bronchial arteries. There was no difference in the appearance of the whole casts or cast-to-lung weight ratios between the two groups, although an incompletely cast lung that was injected through the aorta showed preferential filling of the pleural and peribronchial regions. Nuclear impressions, a sign of good filling, were more frequent and the grade of the images was higher in the group filled through the vena cava. The microvascular density index was not different between the two groups, and a multivariate analysis of variance showed the injection site was not important in determining vascular density. The pulmonary vessels fill nearly as well when injected from the bronchial circulation indicating that the bronchial circulation can immediately perfuse the lung if the pulmonary arteries are obstructed, and casting can be carried out from either side with nearly the same results.
为了确定肺或支气管动脉作为大鼠肺血管铸型注射部位的重要性，我们回顾性比较了一组正常的斯普拉格-道利大鼠，它们分别通过尾静脉和右心室注入肺动脉或通过主动脉和支气管动脉进行铸型。两组之间整体铸型的外观或铸型与肺的重量比没有差异，尽管通过主动脉注射的肺铸型不完全，胸膜和支气管周围区域有优先填充。核印记是填充良好的标志，通过腔静脉填充的组中更频繁出现且图像等级更高。两组之间微血管密度指数没有差异，多因素方差分析表明注射部位对确定血管密度并不重要。从支气管循环注射时，肺血管的填充情况几乎相同，这表明如果肺动脉受阻，支气管循环可以立即灌注肺，并且从两侧进行铸型的结果几乎相同。
