Schraufnagel D E, Schmid A
Department of Medicine, University of Illinois, Chicago 60680.
J Electron Microsc Tech. 1988 Feb;8(2):185-91. doi: 10.1002/jemt.1060080205.
Although techniques for preparing specimens for light and electron microscopy are well established, few studies have sought to establish methods for corrosion casting of the microscopic vessels of the lung. The effects of two concentrations of glutaraldehyde, formaldehyde, and no fixative (saline control) on the filling and structure of the microvascular casts of the lung were studied. All fixatives were infused 5 minutes before casting. Formaldehyde's fixation effects were intermediate between those of glutaraldehyde and no fixation. Leakage of casting material was increased in the animals that were fixed less. Digestion time was prolonged and more undigested tissue was found in the group receiving the concentrated glutaraldehyde, although the difference in neither of these features alone reached statistical significance. Circumferential bands occurred more frequently in the large vessel casts of the less fixed animals, which may account for the less filling microvasculature in these lungs. However, good quality casts were obtained with each method.
尽管用于光镜和电镜标本制备的技术已经成熟，但很少有研究致力于建立肺微血管腐蚀铸型的方法。研究了两种浓度的戊二醛、甲醛以及无固定剂（生理盐水对照）对肺微血管铸型填充和结构的影响。所有固定剂均在铸型前5分钟注入。甲醛的固定效果介于戊二醛和无固定之间。固定较少的动物中铸型材料渗漏增加。接受浓缩戊二醛的组消化时间延长，未消化组织更多，尽管这两个特征单独的差异均未达到统计学意义。在固定较少的动物的大血管铸型中，周向带更频繁出现，这可能是这些肺中微血管填充较少的原因。然而，每种方法都获得了高质量的铸型。
