Silva Manoel Eduardo da, Uriostegui Miguel Angel Mercado, Millán-Orozco Jair, Gives Pedro Mendoza de, Hernández Enrique Liébano, Braga Fabio Ribeiro, Araújo Jackson Victor de
Campo Experimental de Pitangui, Empresa de Pesquisa Agropecuária de Minas Gerais - EPAMIG, Pitangui, MG, Brasil.
Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, Jiutepec, México.
Rev Bras Parasitol Vet. 2017 Jan-Mar;26(1):92-95. doi: 10.1590/S1984-29612016091. Epub 2017 Jan 26.
The purpose of this study was to evaluate the predatory activity of the nematode Butlerius spp. and fungal isolates of Duddingtonia flagrans, Clonostachys rosea, Arthrobotrys musiformis and Trichoderma esau against H. contortus infective larvae (L3) in grass pots. Forty-eight plastic gardening pots containing 140 g of sterile soil were used. Panicum spp. grass seeds (200 mg) were sown into each pot and individually watered with 10 mL of tap water. Twelve days after seeding, the pots were randomly divided into 6 groups (n=8). Two thousand H. contortus infective larvae (L3) were added to each group. Additionally, the following treatments were established: Group 1 - 2000 Butlerius spp. larvae; group 2 - A. musiformis (1x107 conidia); group 3 - T. esau (1x107 conidia); group 4 - C. rosea (1x107 conidia), group 5 - D. flagrans (1x107conidia) and Group 6 - no biological controller (control group). The larval population of H. contortus exposed to Butlerius spp. was reduced by 61.9%. Population reductions of 90.4, 66.7, 61.9 and 85.7% were recorded in the pots containing A. musiformis, T. esau, C. rosea and D. flagrans, respectively. The results of this study indicate that the predatory nematode Butlerius spp. and the assessed fungi display an important predatory activity can be considered suitable potential biological control agents.
本研究的目的是评估线虫巴氏属（Butlerius spp.）以及隔指孢属（Duddingtonia flagrans）、粉红粘帚霉（Clonostachys rosea）、丝状节丛孢（Arthrobotrys musiformis）和埃索木霉（Trichoderma esau）的真菌分离株对草钵中捻转血矛线虫感染性幼虫（L3）的捕食活性。使用了48个装有140克无菌土壤的塑料园艺花盆。将黍属（Panicum spp.）草籽（200毫克）播种到每个花盆中，并用10毫升自来水单独浇水。播种12天后，将花盆随机分为6组（n = 8）。向每组添加2000条捻转血矛线虫感染性幼虫（L3）。此外，设置了以下处理：第1组 - 2000条巴氏属幼虫；第2组 - 丝状节丛孢（1×10⁷分生孢子）；第3组 - 埃索木霉（1×10⁷分生孢子）；第4组 - 粉红粘帚霉（1×10⁷分生孢子），第5组 - 隔指孢（1×10⁷分生孢子）和第6组 - 不使用生物防治剂（对照组）。暴露于巴氏属的捻转血矛线虫幼虫数量减少了61.9%。在含有丝状节丛孢、埃索木霉、粉红粘帚霉和隔指孢的花盆中，幼虫数量分别减少了90.4%、66.7%、61.9%和85.7%。本研究结果表明，捕食性线虫巴氏属和所评估的真菌表现出重要的捕食活性，可被视为合适的潜在生物防治剂。
