Van Winkle Laura S, Kelty Jacklyn S, Plopper Charles G
Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, California.
John Muir Institute for the Environment, Center for Health and the Environment, University of California, Davis, California.
Curr Protoc Toxicol. 2017 Feb 1;71:24.5.1-24.5.26. doi: 10.1002/cptx.18.
This unit focuses on protocols for assessing microenvironment-specific responses in the thoracic lung tissues. Aspects of the entire respiratory system serve as potential targets for candidate toxicants, but each candidate toxicant may impact distinct sites due to differential distribution of either the toxicant or the target cells. Within the conducting airways, the composition of resident cell populations and the metabolic capabilities of the cell populations vary greatly. Thus, studies of this region of the lung require unique, site-selective methods to clearly define the toxic response. Without site-specific sampling, as described in this chapter, the experimental limit of detection for toxicant effects in conducting airways is weakened because differences unrelated to treatment, but related to location, may dominate the response. The protocols included here allow assessment of toxicological responses in the tracheobronchial airways and the gas exchange area of the lung, with specific application to laboratory mammals. © 2017 by John Wiley & Sons, Inc.
本单元重点关注评估胸段肺组织中微环境特异性反应的方案。整个呼吸系统的各个方面都可能成为候选毒物的潜在靶点，但由于毒物或靶细胞的分布差异，每种候选毒物可能会影响不同的部位。在传导气道内，驻留细胞群体的组成和细胞群体的代谢能力差异很大。因此，对肺部该区域的研究需要独特的、位点选择性的方法来明确界定毒性反应。如果没有如本章所述的位点特异性采样，传导气道中毒物效应的实验检测极限就会被削弱，因为与处理无关但与位置有关的差异可能主导反应。这里包含的方案允许评估气管支气管气道和肺部气体交换区域的毒理学反应，并特别适用于实验哺乳动物。© 2017约翰威立国际出版公司
