Luo DanDan, Qu Chao, Zhang ZhenBiao, Xie JianHui, Xu LieQiang, Yang HongMei, Li CaiLan, Lin GuoSheng, Wang HongFeng, Su ZiRen
1 School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, China .
2 Guangdong Province Forestry Science and Technology Extension Station , Guangzhou, China .
J Med Food. 2017 Feb;20(2):180-188. doi: 10.1089/jmf.2016.3827.
Constipation is a common disorder that is a significant source of morbidity among people around the world ranging from 2% to 28%. Dendrobium officinale Kimura et Migo is a traditional herbal medicine and health food used for tonicity of the stomach and promotion of body fluid production in China. This study aimed to prepare the ultrafine powder of Dendrobium officinale (UDO) and investigate its laxative effect and potential mechanism in mice with diphenoxylate-induced constipation. Results indicated that the mean diameter (d) of UDO obtained by ball milling was 6.56 μm. UDO (62.5, 125, and 250 mg/kg, p.o.) could significantly enhance the gastrointestinal transit ratio and promote fecal output. Moreover, UDO treatment resulted in significant increases in the serum levels of acetylcholinesterase (AChE), gastrin (Gas), motilin (MTL), and substance P (SP), and obviously decreased serum contents of somatostatin (SS). Taken together, UDO, which can be easily obtained through milling to a satisfactory particle size, exhibited obvious laxative effect in diphenoxylate-induced constipated mice, and the mechanism might be associated with elevated levels of AChE, Gas, MTL, SP, and reduced production of SS. UDO has the potential for further development into an alternative effective diet therapy for constipation.
便秘是一种常见疾病，是全球发病率在2%至28%的人群中发病的重要原因。铁皮石斛是一种传统草药和保健食品，在中国用于健胃和促进津液生成。本研究旨在制备铁皮石斛超微粉（UDO），并研究其对苯乙哌啶诱导的便秘小鼠的通便作用及潜在机制。结果表明，通过球磨获得的UDO平均直径（d）为6.56μm。UDO（62.5、125和250mg/kg，口服）可显著提高胃肠传输率并促进粪便排出。此外，UDO治疗导致血清乙酰胆碱酯酶（AChE）、胃泌素（Gas）、胃动素（MTL）和P物质（SP）水平显著升高，血清生长抑素（SS）含量明显降低。综上所述，通过研磨可轻松获得令人满意粒径的UDO，在苯乙哌啶诱导的便秘小鼠中表现出明显的通便作用，其机制可能与AChE、Gas、MTL、SP水平升高以及SS生成减少有关。UDO有进一步开发成为便秘替代有效饮食疗法的潜力。
