Chen Qing-Qing, Su Jie, DU Yu-Zhong, Sun Chen-Chen, Chen Su-Hong, Lyu Gui-Yuan
Zhejiang Chinese Medical University Hangzhou 310053, China.
Zhejiang University of Technology Hangzhou 310014, China.
Zhongguo Zhong Yao Za Zhi. 2021 Apr;46(7):1651-1657. doi: 10.19540/j.cnki.cjcmm.20200810.403.
Dendrobium officinale is a traditional Chinese medicine for nourishing Yin and benefiting stomach. Its superfine powder has many advantages, such as good dissolution, high utilization rate, strong integrity and easy to use. However, the researches on effect of D. officinale superfine powder on stomach Yin deficiency model are still not sufficient. In this experiment, we explored the effect of D. officinale superfine powder in mice model with stomach Yin deficiency caused by "spicy overeating", and provided certain reference value for its application in gastrointestinal diseases. Male ICR mice were randomly divided into normal group, model group, Yiweitang group, omeprazole group, and D. officinale superfine powder high, medium and low dose groups. The mixture of wine and pepper liquid was given by gavage administration for 30 d, and the corresponding drug was given for 60 d while the model was conti-nued. The body weight, food intake, water intake, fecal moisture content and particle number, foot temperature of mice were measured. The levels of serum gastrin(Gas), motilin(MTL) and somatostatin(SS) were measured by ELISA. Gastric histomorpho-logy was observed by HE staining. The expression levels of nuclear factor kappa B(NF-κB) and cyclooxygenase-2(COX-2) were determined by immunohistochemistry. The expression levels of B-cell lymphoma-2(Bcl-2) and Bcl-2 associated X protein(Bax) in gastric tissues were detected by Western blot. The results showed that D. officinale superfine powder could increase the food intake, water intake, fecal moisture content and particle number, reduce the foot temperature, improve the pathological changes of gastric mucosa, reduce the expression of NF-κB, COX-2 protein in gastric tissues, and increase the ratio of Bax/Bcl-2. D. officinale superfine powder can "nourish Yin and benefit the stomach", improve the syndrome of stomach Yin deficiency, such as "hunger but not want to eat, dry mouth but not want to drink, hand and feet hot, constipation", and reduce the damage of gastric mucosa. The mechanism may be related to regulating the secretion of gastrointestinal hormones, inhibiting the inflammation of gastric tissues and promoting the apoptosis of abnormal cells in gastric tissues.
铁皮石斛是一种传统的滋阴益胃中药。其超微粉具有溶解性好、利用率高、完整性强、使用方便等诸多优点。然而，关于铁皮石斛超微粉对胃阴虚模型作用的研究仍不充分。本实验探讨了铁皮石斛超微粉对“嗜食辛辣”所致胃阴虚小鼠模型的影响，为其在胃肠道疾病中的应用提供一定参考价值。将雄性ICR小鼠随机分为正常组、模型组、益胃汤组、奥美拉唑组以及铁皮石斛超微粉高、中、低剂量组。通过灌胃给予酒椒液30天，在造模的同时给予相应药物60天。测量小鼠的体重、进食量、饮水量、粪便含水量及颗粒数、足温。采用ELISA法检测血清胃泌素（Gas）、胃动素（MTL）和生长抑素（SS）水平。通过HE染色观察胃组织形态学。采用免疫组化法检测核因子κB（NF-κB）和环氧化酶-2（COX-2）的表达水平。采用Western blot法检测胃组织中B细胞淋巴瘤-2（Bcl-2）和Bcl-2相关X蛋白（Bax）的表达水平。结果表明，铁皮石斛超微粉可增加进食量、饮水量、粪便含水量及颗粒数，降低足温，改善胃黏膜病理变化，降低胃组织中NF-κB、COX-2蛋白表达，提高Bax/Bcl-2比值。铁皮石斛超微粉能“滋阴益胃”，改善胃阴虚证候，如“饥不欲食、口干不欲饮、手足心热、便秘”等，并减轻胃黏膜损伤。其机制可能与调节胃肠激素分泌、抑制胃组织炎症及促进胃组织异常细胞凋亡有关。
