Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2017 May 5;178:1-7. doi: 10.1016/j.saa.2017.01.057. Epub 2017 Jan 27.
In this work, a chemiluminescence (CL) biosensor was prepared for ultrasensitive determination of deoxyribonucleic acid (DNA) based on the adsorption recognition function between core-shell FeO@SiO - graphene oxide (FeO@SiO@GO) polymers and DNA. The FeO@SiO@GO polymers were composed by GO and magnetite nanoparticles. And the core-shell polymers were confirmed by Scanning Electron Microscope (SEM), X-Ray Powder Diffraction (XRD) and Fourier Transform Infrared (FTIR). Then FeO@SiO@GO was modified by DNA. Based on the principle of complementary base, FeO@SiO@GO-DNA was introduced to the CL system and the selectivity, sensitivity of DNA detection was significantly improved. The adsorption properties of FeO@SiO@GO to DNA were researched through the adsorption equilibrium, adsorption kinetic and thermodynamics. Under optimized CL conditions, DNA could be assayed with the linear concentration range of 5.0×10-2.5×10mol/L. The detection limit was 1.7×10mol/L (3δ) and the relative standard deviation (RSD) was 3.1%. The biosensor was finally used for the determination of DNA in laboratory samples and recoveries ranged from 99% to 103%. The satisfactory results revealed the potential application of FeO@SiO@GO-DNA-CL biosensor in the diagnosis and the treatment of human genetic diseases.
在这项工作中，基于核壳型 FeO@SiO2-氧化石墨烯（FeO@SiO@GO）聚合物与 DNA 之间的吸附识别功能，制备了用于超灵敏测定脱氧核糖核酸（DNA）的化学发光（CL）生物传感器。FeO@SiO@GO 聚合物由 GO 和磁铁矿纳米粒子组成。核壳聚合物通过扫描电子显微镜（SEM）、X 射线粉末衍射（XRD）和傅里叶变换红外光谱（FTIR）进行了确认。然后，通过 DNA 对 FeO@SiO@GO 进行了修饰。基于互补碱基的原理，将 FeO@SiO@GO-DNA 引入 CL 体系，显著提高了 DNA 检测的选择性和灵敏度。通过吸附平衡、吸附动力学和热力学研究了 FeO@SiO@GO 对 DNA 的吸附特性。在优化的 CL 条件下，DNA 的测定线性浓度范围为 5.0×10-2.5×10mol/L。检测限为 1.7×10mol/L（3δ），相对标准偏差（RSD）为 3.1%。该生物传感器最终用于实验室样品中 DNA 的测定，回收率范围为 99%至 103%。满意的结果表明，FeO@SiO@GO-DNA-CL 生物传感器在人类遗传疾病的诊断和治疗方面具有潜在的应用价值。
