基于适体复合物的化学发光生物传感器用于凝血酶检测。
A chemiluminescence biosensor for the detection of thrombin based on the aptamer composites.
机构信息
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Mar 5;192:153-158. doi: 10.1016/j.saa.2017.10.074. Epub 2017 Oct 31.
An efficient, rapid, simple and ultrasensitive chemiluminescence (CL) approach was proposed for thrombin detection based on the aptamer-thrombin recognition. The aptamer composites were synthesized in this work using graphene oxide (GO) as the backing material. The thrombin was adsorbed on the aptamer composites based on the aptamer-thrombin recognition. Thus, thrombin could be quantified by the difference value of the CL intensity between supernate of the sample and the mixture which composed of thrombin and coexisted substances. The CL intensity exhibits a stable response to thrombin over a concentration range from 2.5×10 to 1×10mol·L with a detection limit as low as 8.3×10mol·L, the relative standard deviation (RSD) was found to be 4.9% for 11 determinations of 1.25×10mol·L thrombin. Finally, the applicability of the method was verified by applying to serum samples. The recoveries were in the range of 90.3-101.0% with RSD of 2.6-3.2%.
一种基于适体-凝血酶识别的用于凝血酶检测的高效、快速、简单和超灵敏化学发光（CL）方法被提出。在这项工作中，使用氧化石墨烯（GO）作为支撑材料合成了适体复合材料。基于适体-凝血酶识别，凝血酶被吸附在适体复合材料上。因此，通过比较样品上清液和包含凝血酶和共存物质的混合物之间的 CL 强度差值，可以定量检测凝血酶。CL 强度对凝血酶的浓度范围在 2.5×10 到 1×10mol·L 之间表现出稳定的响应，检测限低至 8.3×10mol·L，对于 11 次 1.25×10mol·L 凝血酶的测定，相对标准偏差（RSD）为 4.9%。最后，通过应用于血清样品验证了该方法的适用性。回收率在 90.3-101.0%范围内，RSD 为 2.6-3.2%。
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