Hornbeck Peter, Fleisher Thomas A, Papadopoulos Nicholas M
Cell Signaling Technologies, Danvers, Massachusetts.
Warren Grant Magnuson Clinical Center, Bethesda, Maryland.
Curr Protoc Immunol. 2017 Feb 2;116:2.2.1-2.2.7. doi: 10.1002/cpim.19.
On identifying a new monoclonal antibody, or in characterizing antibodies in sera evoked by disease or immunization, it is particularly informative to determine the serological class of the antibodies. The serological class of the protein is determined by the structure of the antibody constant region. Several methods of class or isotype determination are outlined in this unit: sandwich ELISA, electrophoresis, and immunofixation, or use of a variety of commercially available kits. Different purification schemes and approaches for enzymatic fragmentation of the antibodies depend on the class or isotype of the antibody, so this information streamlines these processes. © 2017 by John Wiley & Sons, Inc.
在鉴定一种新的单克隆抗体时，或者在表征由疾病或免疫接种引起的血清中的抗体时，确定抗体的血清学类别特别有用。蛋白质的血清学类别由抗体恒定区的结构决定。本单元概述了几种类别或同种型测定方法：夹心酶联免疫吸附测定（ELISA）、电泳和免疫固定，或使用各种市售试剂盒。抗体的不同纯化方案和酶切方法取决于抗体的类别或同种型，因此这些信息简化了这些过程。© 2017约翰威立父子公司版权所有
