Weis-Garcia Frances, Carnahan Robert H
Cold Spring Harb Protoc. 2017 Nov 1;2017(11):pdb.prot093831. doi: 10.1101/pdb.prot093831.
For research groups needing to isotype a significant number of antibodies on a fairly regular basis, the protocol outlined here provides a cost-effective option. In this simple sandwich ELISA, the monoclonal antibody is first captured with antibodies that discriminate between heavy-chain isotypes and then is detected with antibodies specific for each light chain. This combination ensures that free light chain secreted by hybridomas does not yield a false-positive result, because in a true positive both the capture and detecting antibodies bind the monoclonal antibody.
对于需要定期对大量抗体进行亚型鉴定的研究小组而言，此处概述的方案提供了一种经济高效的选择。在这种简单的夹心酶联免疫吸附测定法中，首先用能够区分重链亚型的抗体捕获单克隆抗体，然后用针对每条轻链的特异性抗体进行检测。这种组合可确保杂交瘤分泌的游离轻链不会产生假阳性结果，因为在真正的阳性结果中，捕获抗体和检测抗体都会与单克隆抗体结合。
