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检测特异性抗体的双向免疫扩散试验(欧氏试验)

Double-Immunodiffusion Assay for Detecting Specific Antibodies (Ouchterlony).

作者信息

Hornbeck Peter

机构信息

Cell Signaling Technologies, Danvers, Massachusetts.

出版信息

Curr Protoc Immunol. 2017 Feb 2;116:2.3.1-2.3.4. doi: 10.1002/cpim.18.

DOI:10.1002/cpim.18
PMID:28150863
Abstract

The method first described by Ouchterlony in 1948 is a classic and simple technique that permits evaluation and comparison of antibodies in animal or human sera directed against protein or complex carbohydrate antigens. It is a low-tech procedure that may provide information on the relative quantity of antibody activity and the nature of the antigenic epitopes in different preparations. © 2017 by John Wiley & Sons, Inc.

摘要

1948年由奥克特洛尼首次描述的方法是一种经典且简单的技术,可用于评估和比较动物或人血清中针对蛋白质或复合碳水化合物抗原的抗体。这是一种技术要求不高的程序,可提供不同制剂中抗体活性的相对量以及抗原表位性质的相关信息。© 2017约翰威立国际出版公司。

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