Obexer G, Ebner H, Feldner H, Rumpold H, Kraft D
Wien Klin Wochenschr. 1983 Nov 25;95(22):789-91.
Sera from 58 patients with suspected extrinsic allergic alveolitis (EAA) were tested in parallel by the double diffusion test and the "enzyme-linked immunosorbent assay (ELISA)" using 10 different antigen solutions (extracts from thermophilic actinomycetes, various mycetes, pigeon serum, pigeon droppings and the wheat weevil sitophilus granarius). The ELISA technique was more sensitive than the double diffusion test in detecting antibodies to the panel of antigens used. However, by using several antigen dilutions the number of sera positive sera with the double diffusion test increased and an overall correlation of 93.1% with the ELISA was achieved. The results of the study are discussed with regard to the advantages and disadvantages of both test systems for the in vitro diagnosis of EAA.
采用10种不同抗原溶液（嗜热放线菌提取物、各种霉菌、鸽血清、鸽粪和谷象），通过双向扩散试验和“酶联免疫吸附测定（ELISA）”对58例疑似外源性过敏性肺泡炎（EAA）患者的血清进行平行检测。在检测针对所用抗原组的抗体时，ELISA技术比双向扩散试验更敏感。然而，通过使用几种抗原稀释液，双向扩散试验中阳性血清的数量增加，并且与ELISA的总体相关性达到了93.1%。针对这两种检测系统在EAA体外诊断中的优缺点对研究结果进行了讨论。
