College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PRChina.
College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PRChina.
Talanta. 2017 Apr 1;165:136-142. doi: 10.1016/j.talanta.2016.11.051. Epub 2016 Nov 22.
Using sterculia lychnophora seeds as precursors for the first time, fluorescent carbon dots (CDs) were synthesized by simple hydrothermal treatment. The quantum yield of as-synthesized CDs was 6.9% by using quinine sulfate as the reference. The fluorescence of CDs could be effectively quenched by a MnO nanosheet based on fluorescence resonance energy transfer (FRET). Ascorbic acid (AA) could reduce MnO to Mn and result in the destruction of the MnO nanosheets, which could induce the fluorescence recovery of the CDs. In particular, alkaline phosphatase (ALP) could bio-catalyze acid 2-phosphate (AAP) hydrolysis to AA. Here, an efficient fluorescence probe based on a CDs-MnO nanosheet for rapid and selective detection of ALP was reported for the first time. Excellent performance for the detection of ALP was observed with high sensitivity and a detection limit of 0.4U/L owing to the low background. The detection of ALP in human serum was conducted with satisfactory results, demonstrating its potential applications in clinical diagnosis.
首次以乌桕种子为前驱体，通过简单的水热法合成了荧光碳点（CDs）。以硫酸奎宁为参比，合成的 CDs 的量子产率为 6.9%。基于荧光共振能量转移（FRET），MnO 纳米片可有效猝灭 CDs 的荧光。抗坏血酸（AA）可将 MnO 还原为 Mn，并破坏 MnO 纳米片，从而诱导 CDs 的荧光恢复。特别地，碱性磷酸酶（ALP）可生物催化酸 2-磷酸（AAP）水解生成 AA。首次报道了一种基于 CDs-MnO 纳米片的高效荧光探针，用于快速、选择性检测 ALP。由于背景低，检测 ALP 具有很高的灵敏度和检测限（0.4U/L），表现出优异的性能。在人血清中检测 ALP 取得了令人满意的结果，表明其在临床诊断中有潜在的应用。
