Zhang Yunyi, Li Yongxin, Zhang Cuiyun, Zhang Qingfeng, Huang Xinan, Yang Meiding, Shahzad Sohail Anjum, Lo Kenneth Kam-Wing, Yu Cong, Jiang Shichun
School of Materials Science and Engineering, Tianjin University, Tianjin, 300072, China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China.
Anal Bioanal Chem. 2017 Aug;409(20):4771-4778. doi: 10.1007/s00216-017-0420-9. Epub 2017 Jun 14.
A fluorescence turn-on assay for alkaline phosphatase (ALP) activity is developed through the controlled release of polyethyleneimine-capped copper nanoclusters (PEI-capped CuNCs) from the MnO nanosheets. In an aqueous solution, the positively charged PEI-capped CuNCs could be adsorbed onto the surface of the negatively charged MnO nanosheets. Such adsorption through favorable electrostatic interactions could efficiently quench the nanocluster fluorescence emission via resonance energy transfer from the PEI-capped CuNCs to the MnO nanosheets. 2-Phospho-L-ascorbic acid (AAP) could be hydrolyzed to L-ascorbic acid (AA) in the presence of ALP. AA could reduce MnO into Mn and trigger the disintegration of the MnO nanosheets. As a result, the CuNCs were released and the quenched fluorescence was recovered efficiently. The detection strategy is simple, inexpensive, sensitive, selective, with low toxicity, and has better biocompatibility. The newly fabricated biosensor for ALP activity will potentially make it a robust candidate for numerous biological and biomedical applications.
通过从MnO纳米片可控释放聚乙烯亚胺封端的铜纳米簇（PEI封端的CuNCs），开发了一种用于碱性磷酸酶（ALP）活性的荧光开启检测方法。在水溶液中，带正电荷的PEI封端的CuNCs可吸附到带负电荷的MnO纳米片表面。这种通过有利的静电相互作用的吸附可通过从PEI封端的CuNCs到MnO纳米片的共振能量转移有效地猝灭纳米簇荧光发射。在ALP存在下，2-磷酸-L-抗坏血酸（AAP）可水解为L-抗坏血酸（AA）。AA可将MnO还原为Mn并引发MnO纳米片的分解。结果，CuNCs被释放，猝灭的荧光被有效恢复。该检测策略简单、廉价、灵敏、选择性好、毒性低且具有更好的生物相容性。新制备的用于ALP活性的生物传感器有望使其成为众多生物和生物医学应用的有力候选者。
