Department of Analytical and Food Chemistry, Faculty of Chemistry, University of Salamanca, Plaza de la Merced, 37008 Salamanca, Spain.
Food Technology, University of Salamanca, E.P.S. de Zamora, Avenida Requejo 33, 49022 Zamora, Spain.
Talanta. 2017 Apr 1;165:533-539. doi: 10.1016/j.talanta.2016.12.061. Epub 2017 Jan 4.
This study analyses the presence of pesticides in 106 samples of raw propolis from Spain and Chile. The components detected and quantified using gas chromatography mass spectrometry were as follows: triamidefon present in 70.1% of the samples with concentrations between 0.35 and 42.17mg/kg, and dicofol detected in 7.5% of the samples with concentrations between 0.39 and 2.83mg/kg. Diclorofluanid, procymidone, folpet, propham and metazachlor were also detected but only in a small number of samples. Near infrared spectroscopy with a remote reflectance fibre-optic probe was used to detect samples contaminated with triadimefon via partial least squares discriminant, with a R value of 0.71. In addition, the presence of the pesticide triadimefon in propolis was quantified using the modified partial least squares (MPLS) regression method. The calibration results showed a coefficient of determination (R) of 0.81, a root mean square error (RMSE) of 0.36, and a ratio of performance to deviation (RPD) of 2.5.
本研究分析了来自西班牙和智利的 106 份生蜂胶样本中农药的存在情况。使用气相色谱-质谱联用技术检测和定量的成分如下：三唑酮，存在于 70.1%的样本中，浓度在 0.35 至 42.17mg/kg 之间，以及三氯杀螨醇，检测到存在于 7.5%的样本中，浓度在 0.39 至 2.83mg/kg 之间。还检测到了敌菌丹、咯菌腈、福美双、异丙威和甲草胺，但只在少数样本中检测到。使用带有远程反射光纤探头的近红外光谱法通过偏最小二乘判别法检测到受三唑酮污染的样品，R 值为 0.71。此外，还使用改进的偏最小二乘（MPLS）回归法对蜂胶中农药三唑酮的含量进行了定量。校准结果显示，决定系数（R）为 0.81，均方根误差（RMSE）为 0.36，性能偏差比（RPD）为 2.5。
