Bundesanstalt für Materialforschung und -prüfung (BAM), 12205 Berlin, Germany; School of Analytical Sciences Adlershof (SALSA), Humboldt-Universität zu Berlin, Unter den Linden 6, 10099 Berlin, Germany.
Bundesanstalt für Materialforschung und -prüfung (BAM), 12205 Berlin, Germany; Technische Universität Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany.
Talanta. 2017 Apr 1;165:619-624. doi: 10.1016/j.talanta.2017.01.004. Epub 2017 Jan 4.
This study focused on quantitative detection of cocaine on Euro banknotes in Germany. A sensitive direct competitive immunoassay was developed and optimized with a limit of detection (LOD) of 5.6ng/L. Exhaustive cocaine extraction by solvent was tested using different methanol concentrations and buffered solutions. Cross-reactivity studies were performed to determine the degree of interference of cocaine metabolites with the immunoassay. Sixty-five Euro banknotes obtained from different districts in Berlin were evaluated. A 100% contamination frequency with cocaine was detected. A comparison between the amount of cocaine extracted by cotton swabbing of one square centimeter of the banknote showed a good correlation for lower contamination levels. This assay showed high sensitivity of detecting pg of cocaine per 1cm of one banknote by swabbing 1cm: 0, 14, and 21pg/cm. Moreover, three notes of different denominations revealed high cocaine concentration; 1.1mg/note, and twice 55µg/note.
本研究专注于对德国欧元纸币上可卡因的定量检测。开发并优化了一种具有 5.6ng/L 检测限（LOD）的灵敏直接竞争免疫分析法。通过使用不同的甲醇浓度和缓冲溶液，对可卡因进行了彻底的溶剂萃取。进行了交叉反应研究，以确定可卡因代谢物对免疫分析法的干扰程度。评估了从柏林不同地区获得的 65 张欧元纸币。检测到可卡因的污染频率为 100%。通过对纸币一平方厘米的棉签擦拭提取可卡因的量进行比较，对于较低的污染水平，显示出良好的相关性。通过棉签擦拭 1cm，该检测法对每 1cm 的纸币上 pg 级的可卡因显示出很高的灵敏度：0、14 和 21pg/cm。此外，三张不同面额的纸币显示出高浓度的可卡因；每张 1.1 毫克，两张 55 微克。
