Yuan Zhuning, Chen Yaosheng, Zhang Xumeng, Zhou Xingyu, Li Mingsen, Chen Hu, Wu Ming, Zhang Ying, Mo Delin
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China.
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China.
Biochem Biophys Res Commun. 2017 Mar 11;484(3):592-597. doi: 10.1016/j.bbrc.2017.01.143. Epub 2017 Jan 31.
Myotubularin related protein 7 (MTMR7) is a key member of the highly conserved myotubularin related proteins (MTMRs) family, which has phosphatase activity. MTMR7 was increased during myoblast differentiation and exhibited high expression level at primary fibers formation stages in pigs. This suggests that MTMR7 may be involved in myogenesis. In our study, we investigated the roles of MTMR7 on proliferation and differentiation of C2C12 myoblasts. Knocking down MTMR7 not only enhanced myoblast early differentiation via altering the expression of Myf5, but also promoted myoblast proliferation through increasing cyclinA2 expression. The improved proliferation capacity was related to the increased phosphorylation of AKT. Taken together, our research demonstrates that MTMR7 plays an important role in proliferation and early differentiation of C2C12 myoblast.
肌管素相关蛋白7（MTMR7）是高度保守的肌管素相关蛋白（MTMRs）家族的关键成员，该家族具有磷酸酶活性。在猪的成肌细胞分化过程中，MTMR7表达增加，并且在初级纤维形成阶段表现出高表达水平。这表明MTMR7可能参与了肌生成过程。在我们的研究中，我们探究了MTMR7对C2C12成肌细胞增殖和分化的作用。敲低MTMR7不仅通过改变Myf5的表达增强了成肌细胞的早期分化，还通过增加细胞周期蛋白A2的表达促进了成肌细胞的增殖。增殖能力的提高与AKT磷酸化增加有关。综上所述，我们的研究表明MTMR7在C2C12成肌细胞的增殖和早期分化中起重要作用。
