Cadoret Véronique, Frapsauce Cynthia, Jarrier Peggy, Maillard Virginie, Bonnet Agnès, Locatelli Yann, Royère Dominique, Monniaux Danielle, Guérif Fabrice, Monget Philippe
PRCUMR85, INRA, CNRS, IFCE, Université de Tours, Nouzilly, France
CHU BretonneauMédecine et Biologie de la Reproduction-CECOS, Tours, France.
Reproduction. 2017 May;153(5):493-508. doi: 10.1530/REP-16-0627. Epub 2017 Feb 2.
In this study, we systematically compared the morphological, functional and molecular characteristics of granulosa cells and oocytes obtained by a three-dimensional model of ovine ovarian follicular growth with those of follicles recovered Preantral follicles of 200 µm diameter were recovered and cultured up to 950 µm over a 20-day period. Compared with follicles, the culture conditions maintained follicle survival, with no difference in the rate of atresia. However, the conditions induced a slight decrease in oocyte growth rate, delayed antrum formation and increased granulosa cell proliferation rate, accompanied by an increase and decrease in and mRNA expression respectively. These changes were associated with advanced granulosa cell differentiation in early antral follicles larger than 400 µm diameter, regardless of the presence or absence of FSH, as indicated by an increase in estradiol secretion, together with decreased AMH secretion and expression, as well as increased expression of , , , , , , , and There was a decrease in the expression of oocyte-specific molecular markers , , , and compared to that Moreover, a higher percentage of the oocytes recovered from cultured follicles 550 to 950 µm in diameter was able to reach the metaphase II meiosis stage. Overall, this model of ovarian follicle development is characterized by accelerated follicular maturation, associated with improved developmental competence of the oocyte, compared to follicles recovered .
在本研究中，我们系统地比较了通过绵羊卵泡生长三维模型获得的颗粒细胞和卵母细胞的形态、功能及分子特征与回收卵泡的相关特征。回收直径为200μm的腔前卵泡，并在20天内将其培养至950μm。与回收卵泡相比，培养条件维持了卵泡存活，闭锁率无差异。然而，这些条件导致卵母细胞生长速率略有下降、卵泡腔形成延迟以及颗粒细胞增殖速率增加，同时分别伴随着mRNA表达的增加和减少。这些变化与直径大于400μm的早期有腔卵泡中颗粒细胞的高级分化相关，无论是否存在促卵泡生成素（FSH），这表现为雌二醇分泌增加，同时抗苗勒管激素（AMH）分泌和表达减少，以及、、、、、、、和表达增加。与相比，卵母细胞特异性分子标记、、、和的表达减少。此外，从直径550至950μm的培养卵泡中回收的卵母细胞能够达到减数分裂中期II阶段的比例更高。总体而言，与回收卵泡相比，这种卵巢卵泡发育模型的特征是卵泡成熟加速，同时卵母细胞的发育能力提高。
