Department of Periodontology, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA.
Cell Therapy Institute, Center for Collaborative Research, Nova Southeastern University, Fort Lauderdale, FL, USA.
Clin Oral Investig. 2017 Nov;21(8):2619-2627. doi: 10.1007/s00784-017-2063-9. Epub 2017 Feb 2.
Platelet rich plasma (PRP) has been utilized in regenerative dentistry as a supra-physiological concentrate of autologous growth factors capable of stimulating tissue regeneration. Despite this, concerns have been expressed regarding the use of anti-coagulants, agents known to inhibit wound healing. In this study, a liquid formulation of platelet rich fibrin (PRF) termed injectable-PRF (i-PRF) without the use of anti-coagulants was investigated.
Standard PRP and i-PRF (centrifuged at 700 rpm (60G) for 3 min) were compared for growth factor release up to 10 days (8 donor samples). Furthermore, fibroblast biocompatibility at 24 h (live/dead assay); migration at 24 h; proliferation at 1, 3, and 5 days, and expression of PDGF, TGF-β, and collagen1 at 3 and 7 days were investigated.
Growth factor release demonstrated that in general PRP had higher early release of growth factors whereas i-PRF showed significantly higher levels of total long-term release of PDGF-AA, PDGF-AB, EGF, and IGF-1 after 10 days. PRP showed higher levels of TGF-β1 and VEGF at 10 days. While both formulations exhibited high biocompatibility and higher fibroblast migration and proliferation when compared to control tissue-culture plastic, i-PRF induced significantly highest migration whereas PRP demonstrated significantly highest cellular proliferation. Furthermore, i-PRF showed significantly highest mRNA levels of TGF-β at 7 days, PDGF at 3 days, and collagen1 expression at both 3 and 7 days when compared to PRP.
i-PRF demonstrated the ability to release higher concentrations of various growth factors and induced higher fibroblast migration and expression of PDGF, TGF-β, and collagen1. Future animal research is now necessary to further validate the use of i-PRF as a bioactive agent capable of stimulating tissue regeneration.
The findings from the present study demonstrate that a potent formulation of liquid platelet concentrates could be obtained without use of anti-coagulants.
富含血小板的血浆（PRP）已被用于再生牙科领域，作为一种超生理浓度的自体生长因子，能够刺激组织再生。尽管如此，人们还是对使用抗凝剂表示了担忧，因为抗凝剂已知会抑制伤口愈合。在这项研究中，我们研究了一种无需使用抗凝剂的液体形式的富血小板纤维蛋白（PRF），称为可注射富血小板纤维蛋白（i-PRF）。
比较了标准 PRP 和 i-PRF（以 700rpm（60G）离心 3 分钟）在 10 天内（8 个供体样本）的生长因子释放情况。此外，还在 24 小时（死活检测）进行了成纤维细胞生物相容性；24 小时迁移；1、3 和 5 天增殖，以及 3 和 7 天 PDGF、TGF-β 和胶原蛋白 1 的表达。
生长因子释放表明，一般来说 PRP 早期释放生长因子的能力较高，而 i-PRF 在 10 天后表现出显著更高水平的 PDGF-AA、PDGF-AB、EGF 和 IGF-1 的总长期释放。PRP 在 10 天时表现出更高水平的 TGF-β1 和 VEGF。虽然这两种制剂与对照组织培养塑料相比表现出高度的生物相容性和更高的成纤维细胞迁移和增殖能力，但 i-PRF 诱导的迁移显著更高，而 PRP 则表现出显著更高的细胞增殖。此外，与 PRP 相比，i-PRF 在第 7 天表现出最高的 TGF-βmRNA 水平，在第 3 天表现出最高的 PDGF 水平，在第 3 天和第 7 天表现出最高的胶原蛋白 1 表达水平。
i-PRF 表现出释放各种生长因子的更高浓度的能力，并诱导更高的成纤维细胞迁移和 PDGF、TGF-β 和胶原蛋白 1 的表达。现在需要进行进一步的动物研究，以进一步验证 i-PRF 作为一种能够刺激组织再生的生物活性剂的使用。
本研究的结果表明，可以在不使用抗凝剂的情况下获得一种有效的液体血小板浓缩剂制剂。
