Han Peilin, Cui Qingbo, Yang Shulong, Wang Hao, Gao Peng, Li Zhaozhu
Pediatric Surgery Department, the Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Biotechnol Lett. 2017 May;39(5):711-719. doi: 10.1007/s10529-017-2296-3. Epub 2017 Feb 2.
To investigate the effects of tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) on the proliferation and differentiation of tendon-derived stem cells (TDSC).
TNF-α inhibits the proliferation and tenogenic/osteogenic differentiation of TDSC but, after simultaneous or sequential treatment with TGF-β1 and TNF-α, the expression of tenogenic/osteogenic-related marker and proliferation of TDSC was significantly increased. During these processes, Smad2/3 and Smad1/5/8 were highly phosphorylated, meaning that the TGF-β and BMP signaling pathways were highly activated. Further study revealed that the expression of Inhibitor-Smad appeared to be negatively correlated to the proliferation and differentiation of TDSC.
Combining the use of TNF-α and TGF-β1 could improve the proliferation and differentiation of TDSC in vitro, and the expression of I-Smad is negatively correlated with TDSC proliferation and differentiation.
研究肿瘤坏死因子-α（TNF-α）和转化生长因子-β1（TGF-β1）对肌腱来源干细胞（TDSC）增殖和分化的影响。
TNF-α抑制TDSC的增殖和肌腱/成骨分化，但在TGF-β1与TNF-α同时或序贯处理后，肌腱/成骨相关标志物的表达及TDSC的增殖显著增加。在这些过程中，Smad2/3和Smad1/5/8高度磷酸化，这意味着TGF-β和骨形态发生蛋白（BMP）信号通路被高度激活。进一步研究表明，抑制性Smad（I-Smad）的表达似乎与TDSC的增殖和分化呈负相关。
联合使用TNF-α和TGF-β1可改善TDSC在体外的增殖和分化，且I-Smad的表达与TDSC的增殖和分化呈负相关。
