肿瘤坏死因子-α和转化生长因子-β1促进肌腱来源干细胞在体外的分化和增殖。
Tumor necrosis factor-α and transforming growth factor-β1 facilitate differentiation and proliferation of tendon-derived stem cells in vitro.
作者信息
Han Peilin, Cui Qingbo, Yang Shulong, Wang Hao, Gao Peng, Li Zhaozhu
机构信息
Pediatric Surgery Department, the Second Affiliated Hospital of Harbin Medical University, Harbin, China.
出版信息
Biotechnol Lett. 2017 May;39(5):711-719. doi: 10.1007/s10529-017-2296-3. Epub 2017 Feb 2.
OBJECTIVES
To investigate the effects of tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) on the proliferation and differentiation of tendon-derived stem cells (TDSC).
RESULTS
TNF-α inhibits the proliferation and tenogenic/osteogenic differentiation of TDSC but, after simultaneous or sequential treatment with TGF-β1 and TNF-α, the expression of tenogenic/osteogenic-related marker and proliferation of TDSC was significantly increased. During these processes, Smad2/3 and Smad1/5/8 were highly phosphorylated, meaning that the TGF-β and BMP signaling pathways were highly activated. Further study revealed that the expression of Inhibitor-Smad appeared to be negatively correlated to the proliferation and differentiation of TDSC.
CONCLUSIONS
Combining the use of TNF-α and TGF-β1 could improve the proliferation and differentiation of TDSC in vitro, and the expression of I-Smad is negatively correlated with TDSC proliferation and differentiation.
目的
研究肿瘤坏死因子-α（TNF-α）和转化生长因子-β1（TGF-β1）对肌腱来源干细胞（TDSC）增殖和分化的影响。
结果
TNF-α抑制TDSC的增殖和肌腱/成骨分化，但在TGF-β1与TNF-α同时或序贯处理后，肌腱/成骨相关标志物的表达及TDSC的增殖显著增加。在这些过程中，Smad2/3和Smad1/5/8高度磷酸化，这意味着TGF-β和骨形态发生蛋白（BMP）信号通路被高度激活。进一步研究表明，抑制性Smad（I-Smad）的表达似乎与TDSC的增殖和分化呈负相关。
结论
联合使用TNF-α和TGF-β1可改善TDSC在体外的增殖和分化，且I-Smad的表达与TDSC的增殖和分化呈负相关。
Zotero 插件