Ciuffo M, Nerva L, Turina M
Istituto per la Protezione Sostenibile delle Piante, CNR, Strada delle Cacce 73, 10135, Turin, Italy.
Arch Virol. 2017 May;162(5):1419-1422. doi: 10.1007/s00705-017-3237-0. Epub 2017 Feb 2.
The complete genome sequence of melon severe mosaic virus (MSMV), genus Tospovirus, family Bunyaviridae, was determined. The small segment is 3283 nucleotide (nt) long and contains two open reading frames in an ambisense organization. The medium segment is 4873 nt long and also encodes two proteins in an ambisense organization. The large segment is 9811 nt long and contains a single, negative-sense ORF. Phylogenetic analysis of each of the five encoded proteins compared to those of tospoviruses present in the databases reveals the same topology for each tree, suggesting that the MSMV genome did not result from recombination or reassortment. Sequence variants present in the RNA population of an infected leaf are described.
确定了番茄斑萎病毒属（Tospovirus）、布尼亚病毒科（Bunyaviridae）甜瓜严重花叶病毒（MSMV）的全基因组序列。小片段长3283个核苷酸（nt），以双义结构包含两个开放阅读框。中片段长4873 nt，也以双义结构编码两种蛋白质。大片段长9811 nt，包含一个单义负链开放阅读框。将编码的五种蛋白质中的每一种与数据库中存在的番茄斑萎病毒的蛋白质进行系统发育分析，结果显示每棵树的拓扑结构相同，这表明MSMV基因组并非重组或重配的结果。描述了感染叶片RNA群体中存在的序列变体。
