Becker S, Kiessling R, Lee N, Klein G
J Natl Cancer Inst. 1978 Dec;61(6):1495-8.
On the basis of studies indicating that natural killer (NK) cells of the mouse can selectively kill certain syngenetic, allogeneic, and xenogeneic tumor cells in short-term Cr release assays and that cell lines established in vitro are more sensitive than the corresponding ascites tumor cells passaged in vivo, the kinetics of the modulation to increased sensitivity was studied after in vitro explanation of the A/Sn mouse-derived YAC ascites lymphoma. Sensitivity to NK lysis appeared after 3 weeks of culturing and reached the level of the continuously cultured line after 2 months. With the more sensitive competition assay, a change could be demonstrated as early as 2--24 hours of culture. The expression of the Moloney murine leukemia virus-determined, cell-surface antigen, measured by quantitative absorption with intact cells, increased in parallel with the NK sensitivity. In contrast, the H-2 alloantigen concentration decreased during in vitro culture.
基于多项研究表明，在短期铬释放试验中，小鼠的自然杀伤（NK）细胞能够选择性地杀伤某些同基因、异基因和异种肿瘤细胞，且体外建立的细胞系比体内传代的相应腹水肿瘤细胞更敏感，因此对源自A/Sn小鼠的YAC腹水淋巴瘤进行体外传代后，研究了其敏感性增加的调节动力学。培养3周后出现对NK裂解的敏感性，并在2个月后达到连续培养细胞系的水平。通过更灵敏的竞争试验，早在培养2 - 24小时就能证明有变化。通过完整细胞的定量吸附测定的莫洛尼鼠白血病病毒决定的细胞表面抗原的表达与NK敏感性平行增加。相反，在体外培养期间H-2同种异体抗原浓度降低。
