Hansson M, Bakacs K K, Kiessling R, Klein G
J Immunol. 1978 Jul;121(1):6-12.
Natural killer (NK) cells can kill certain syngeneic, allogeneic and xenogeneic tumor targets in short-term 51Cr release assays. In the present study, intra- and interspecies NK activity was analyzed. Ten mouse and five human tissue culture lines were used. In direct cytolytic assays with mouse spleen cells or human PBL effectors, intraspecies was much stronger than interspecies reactivity, as a rule. A certain interspecies activity was obtained, stronger in mouse anti-human (M alpha H) than in human anti-mouse (H alpha M) combinations. In the H alpha M system, activity was associated with the same type of PBL-derived non-B-non-T cell fraction as in the intraspecies H alpha H system. The non-B-non-T cell nature of the M alpha H killer cell has been demonstrated previously. Nonlabeled tumor cells were allowed to compete with isotope-labeled targets in intra- and interspecies cytolytic NK tests. NK-sensitive tumor lines of the same species were superior to xenogeneic competitors in both M alpha M and H alpha H tests. In the M alpha M assay, the competing ability of the same human tumors varied, depending on the genotype of the mouse effector cells. None of the human lines tested competed effectively with strain CBA effectors but some showed a certain competition with C57BL effectors. In the H alpha H assay, strong competition was seen with two of the 10 xenogeneic mouse tumors tested.
在短期的51Cr释放试验中，自然杀伤（NK）细胞能够杀伤某些同基因、异基因和异种的肿瘤靶细胞。在本研究中，分析了种内和种间的NK活性。使用了10种小鼠和5种人类组织培养细胞系。通常，在以小鼠脾细胞或人类外周血淋巴细胞（PBL）作为效应细胞的直接细胞溶解试验中，种内反应比种间反应要强得多。获得了一定程度的种间活性，在小鼠抗人（MαH）组合中比在人抗小鼠（HαM）组合中更强。在HαM系统中，活性与种内HαH系统中相同类型的源自PBL的非B非T细胞组分相关。MαH杀伤细胞的非B非T细胞性质先前已得到证实。在种内和种间细胞溶解NK试验中，未标记的肿瘤细胞被允许与同位素标记的靶细胞竞争。在MαM和HαH试验中，同一物种的NK敏感肿瘤细胞系比异种竞争者更具优势。在MαM试验中，相同人类肿瘤的竞争能力有所不同，这取决于小鼠效应细胞的基因型。所测试的人类细胞系中，没有一个能与CBA品系的效应细胞有效竞争，但有些与C57BL品系的效应细胞表现出一定的竞争。在HαH试验中，在所测试的10种异种小鼠肿瘤中有两种表现出强烈的竞争。
