猪免疫球蛋白G对初乳前、哺乳和断奶仔猪丝裂原诱导的淋巴细胞反应的体外调节作用。

In vitro modulating effects of porcine immunoglobulin G on mitogens-induced lymphocyte response in precolostral, suckling and weaned piglets.

作者信息

Valpotić I, Gerencer M, Basić I

机构信息

Pig Breeding Center, Veterinary Faculty, University of Zagreb, Yugoslavia.

出版信息

Vet Immunol Immunopathol. 1989 Sep;22(2):113-22. doi: 10.1016/0165-2427(89)90054-8.

DOI:10.1016/0165-2427(89)90054-8

PMID:2815577

Abstract

The influence of allogeneic IgG on in vitro reactivity of peripheral blood lymphocytes (PBL) of neonatal colostrum-deprived piglets as well as of suckling and weaned piglets was studied. PBL were preincubated with purified allogeneic IgG for 24 h before their ability to respond to PHA, Con A or PWM was tested. PBL of precolostral piglets pretreated with allogeneic IgG exhibited higher response to PHA (P less than 0.01) than untreated control cells. An increased response of PBL treated with IgG was also observed in suckling piglets as compared to their respective control cells (P less than 0.01). Responsiveness of PBL treated with IgG to PWM was suppressed. No differences in response to Con A regardless of the sources of lymphocytes was observed as compared to IgG untreated controls. The results suggest that pretreatment of lymphocytes of piglets with allogeneic IgG modulates their reactivity to mitogens, suppressing the response to PWM and stimulating the response to PHA, respectively.

摘要

研究了同种异体IgG对新生期未摄入初乳仔猪以及哺乳仔猪和断奶仔猪外周血淋巴细胞（PBL）体外反应性的影响。在测试PBL对PHA、Con A或PWM的反应能力之前，先将其与纯化的同种异体IgG预孵育24小时。用同种异体IgG预处理的初乳前仔猪的PBL对PHA的反应（P<0.01）高于未处理的对照细胞。与各自的对照细胞相比，哺乳仔猪中用IgG处理的PBL的反应也有所增加（P<0.01）。用IgG处理的PBL对PWM的反应性受到抑制。与未用IgG处理的对照相比，无论淋巴细胞来源如何，对Con A的反应均未观察到差异。结果表明，用同种异体IgG预处理仔猪的淋巴细胞可调节其对有丝分裂原的反应性，分别抑制对PWM的反应并刺激对PHA的反应。