Preisler H D, Jacobs S K
J Natl Cancer Inst. 1979 Jan;62(1):133-7.
Normal murine spleen cells were sensitized to syngeneic myeloid leukemia cells by RNA extracted from the lymph nodes and spleens of Hartley guinea pigs immunized with the murine leukemia cells. Sensitization mediated by RNA was an active process that required physiologic temperature and at least a 10-minute incubation. RNA extracted from unimmunized guinea pigs of guinea pigs immunized with normal spleen cells failed to sensitize the mouse spleen cells. Sensitization was specifically directed toward leukemia cells, whereas the spleen cells remained unreactive toward normal spleen or bone marrow cells. The sensitizing moiety was RNA itself inasmuch as it was inactivated by RNase and not by DNase or pronase. Preparations whose RNA patterns on sucrose density centrifugation gave evidence of degradation of the RNA did not sensitize normal spleen cells. These studies demonstrate that xenogeneic immune RNA can specifically sensitize normal spleen cells to syngeneic myeloid leukemia cells.
用从小鼠白血病细胞免疫的Hartley豚鼠的淋巴结和脾脏中提取的RNA，使正常小鼠脾细胞对同基因骨髓白血病细胞致敏。RNA介导的致敏是一个活跃的过程，需要生理温度和至少10分钟的孵育。用正常脾细胞免疫的豚鼠或未免疫的豚鼠提取的RNA不能使小鼠脾细胞致敏。致敏作用是特异性针对白血病细胞的，而脾细胞对正常脾细胞或骨髓细胞仍无反应。致敏部分是RNA本身，因为它被核糖核酸酶灭活，而不被脱氧核糖核酸酶或链霉蛋白酶灭活。蔗糖密度离心时RNA图谱显示RNA降解的制剂不能使正常脾细胞致敏。这些研究表明，异种免疫RNA能使正常脾细胞对同基因骨髓白血病细胞产生特异性致敏。
