大鼠肝脏中细胞色素P-450j（IIE1）和P-450b（IIB1）的丙酮依赖性调节
Acetone-dependent regulation of cytochrome P-450j (IIE1) and P-450b (IIB1) in rat liver.
作者信息
Ronis M J, Ingelman-Sundberg M
机构信息
Department of Physiological Chemistry, Karolinska Institute, Stockholm, Sweden.
出版信息
Xenobiotica. 1989 Oct;19(10):1161-5. doi: 10.3109/00498258909043168.
PMID:2815820
Abstract
- Concomitant changes in the concentration of P450 IIB1 (P-450b) and the rate of O-depentylation of 7-pentoxyresorufin was observed in rat liver microsomes after a single intragastric dose of acetone. 2. In contrast the concentration of P450 IIE1 (P-450j) did not coincide with changes in the rate of P450 IIE1-dependent p-nitrophenol hydroxylation or metabolic activation of carbon tetrachloride. 3. Quantification of the proteins in lysosomes indicated that both P450 IIB1 and P450 IIE1 are degraded via an autophagosomal/autolysosomal pathway. 4. It is concluded that P450 IIE1 is catalytically inactivated in microsomes prior to the degradation of this protein.
摘要
- 单次灌胃给予丙酮后，在大鼠肝脏微粒体中观察到细胞色素P450 IIB1（P - 450b）浓度与7 - 戊氧基试卤灵O - 脱戊基化速率的伴随变化。2. 相比之下，细胞色素P450 IIE1（P - 450j）的浓度与细胞色素P450 IIE1依赖性对硝基苯酚羟化速率或四氯化碳的代谢活化变化不一致。3. 溶酶体中蛋白质的定量分析表明，细胞色素P450 IIB1和细胞色素P450 IIE1均通过自噬体/自溶酶体途径降解。4. 得出的结论是，细胞色素P450 IIE1在该蛋白质降解之前在微粒体中被催化失活。
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