Iannella Giannicola, Di Gioia Cira, Carletti Raffaella, Magliulo Giuseppe
Sensory Organs Department.
Radiological, Oncological and Pathological Department, Sapienza University of Rome, Rome, Italy.
Laryngoscope. 2017 Aug;127(8):E283-E290. doi: 10.1002/lary.26458. Epub 2017 Feb 3.
OBJECTIVES/HYPOTHESIS: This study investigates the immunohistochemical expression of vascular endothelial growth factor (VEGF) and CD34 in patients treated for middle ear and mastoid cholesterol granulomas to evaluate the angiogenesis and vascularization of this type of lesion. A correlation between the immunohistochemical data and the radiological and intraoperative evidence of temporal bone marrow invasion and blood source connection was performed to validate this hypothesis.
Retrospective study.
Immunohistochemical expression of VEGF and CD34 in a group of 16 patients surgically treated for cholesterol granuloma was examined. Middle ear cholesteatomas with normal middle ear mucosa and external auditory canal skin were used as the control groups. The radiological and intraoperative features of cholesterol granulomas were also examined.
In endothelial cells, there was an increased expression of angiogenetic growth factor receptors in all the cholesterol granulomas in this study. The quantitative analysis of VEGF showed a mean value of 37.5, whereas the CD34 quantitative analysis gave a mean value of 6.8. Seven patients presented radiological or intraoperative evidence of bone marrow invasion, hematopoietic potentialities, or blood source connections that might support the bleeding theory. In all of these cases there was computed tomography or intraoperative evidence of bone erosion of the middle ear and/or temporal bone structures. The mean values of VEGF and CD34 were 41.1 and 7.7, respectively.
High values of VEGF and CD34 are present in patients with cholesterol granulomas. Upregulation of VEGF and CD34 is indicative of a remarkable angiogenesis and a widespread vascular concentration in cholesterol granulomas.
3b. Laryngoscope, 127:E283-E290, 2017.
目的/假设：本研究调查接受中耳和乳突胆固醇性肉芽肿治疗的患者中血管内皮生长因子（VEGF）和CD34的免疫组化表达，以评估这类病变的血管生成和血管化情况。对免疫组化数据与颞骨骨髓侵犯及血源连接的放射学和术中证据进行相关性分析，以验证该假设。
回顾性研究。
检测一组16例接受胆固醇性肉芽肿手术治疗患者的VEGF和CD34免疫组化表达。以中耳黏膜和外耳道皮肤正常的中耳胆脂瘤作为对照组。还检查了胆固醇性肉芽肿的放射学和术中特征。
在内皮细胞中，本研究中所有胆固醇性肉芽肿的血管生成生长因子受体表达均增加。VEGF定量分析显示平均值为37.5，而CD34定量分析平均值为6.8。7例患者有放射学或术中证据表明存在骨髓侵犯、造血潜能或血源连接，这可能支持出血理论。在所有这些病例中，计算机断层扫描或术中均有中耳和/或颞骨结构骨质侵蚀的证据。VEGF和CD34的平均值分别为41.1和7.7。
胆固醇性肉芽肿患者中VEGF和CD34值较高。VEGF和CD34的上调表明胆固醇性肉芽肿中有显著的血管生成和广泛的血管聚集。
3b。《喉镜》，127:E283-E290，2017年。
