Department of Chemistry, Faculty of Engineering, Universidad Católica Andrés Bello, Caracas, Venezuela.
Department of Analytical Sciences, Faculty of Sciences, National University of Distance, 28040 Madrid, Spain.
Food Chem. 2017 Jun 1;224:432-438. doi: 10.1016/j.foodchem.2016.11.097. Epub 2016 Nov 22.
The aim of this study was to prepare molecularly imprinted polymers (MIPs) with ampicillin (AMP) and to evaluate the feasibility of these materials for being used as solid phase extraction sorbent for the selective preconcentration and determination of AMP in cow milk samples. MIPs were synthesized by bulk polymerization using methacrylic acid or methyl methacrylate as monomer and ethylene glycol dimethacrylate as cross-linker at different ratios. Characterization of the MIPs were carried out by Fourier-transform infrared spectrometry. The variables affecting the molecularly imprinted solid-phase extraction (MISPE) procedure were optimized. AMP recoveries were higher than 98%, and RSD less than 7%. A preconcentration factor of 20 was reached, which was sufficient to determine AMP at levels allowed by the EU (4μgkg) in cow milk. The selectivity of the AMP-MIP was evaluated in presence of other structurally related β-lactam antibiotics (amoxicillin, oxacillin, penicillin G).
本研究旨在制备氨苄青霉素（AMP）的分子印迹聚合物（MIP），并评估这些材料作为固相萃取吸附剂用于选择性预浓缩和牛奶样品中 AMP 测定的可行性。MIP 是通过本体聚合合成的，使用甲基丙烯酸或甲基丙烯酸甲酯作为单体，乙二醇二甲基丙烯酸酯作为交联剂，比例不同。通过傅里叶变换红外光谱对 MIP 进行了表征。优化了影响分子印迹固相萃取（MISPE）过程的变量。AMP 的回收率高于 98%，RSD 小于 7%。达到了 20 倍的浓缩因子，足以在欧盟允许的水平（4μgkg）下检测牛奶中的 AMP。在存在其他结构相关的β-内酰胺抗生素（阿莫西林、苯唑西林、青霉素 G）的情况下，评估了 AMP-MIP 的选择性。
