• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于适配体的共轭识别技术在食品中小分子检测方面的进展。

Advances in Aptamer-Based Conjugate Recognition Techniques for the Detection of Small Molecules in Food.

作者信息

Deng Xin, Ma Biao, Gong Yunfei, Li Jiali, Zhou Yuxin, Xu Tianran, Hao Peiying, Sun Kai, Lv Zhiyong, Yu Xiaoping, Zhang Mingzhou

机构信息

Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, China.

Hangzhou Quickgene Sci-Tech. Co., Ltd., Hangzhou 310018, China.

出版信息

Foods. 2024 Jun 2;13(11):1749. doi: 10.3390/foods13111749.

DOI:10.3390/foods13111749
PMID:38890976
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11172347/
Abstract

Small molecules are significant risk factors for causing food safety issues, posing serious threats to human health. Sensitive screening for hazards is beneficial for enhancing public security. However, traditional detection methods are unable to meet the requirements for the field screening of small molecules. Therefore, it is necessary to develop applicable methods with high levels of sensitivity and specificity to identify the small molecules. Aptamers are short-chain nucleic acids that can specifically bind to small molecules. By utilizing aptamers to enhance the performance of recognition technology, it is possible to achieve high selectivity and sensitivity levels when detecting small molecules. There have been several varieties of aptamer target recognition techniques developed to improve the ability to detect small molecules in recent years. This review focuses on the principles of detection platforms, classifies the conjugating methods between small molecules and aptamers, summarizes advancements in aptamer-based conjugate recognition techniques for the detection of small molecules in food, and seeks to provide emerging powerful tools in the field of point-of-care diagnostics.

摘要

小分子是引发食品安全问题的重要风险因素，对人类健康构成严重威胁。对危害进行灵敏筛查有利于加强公共安全。然而，传统检测方法无法满足小分子现场筛查的要求。因此，有必要开发具有高灵敏度和特异性的适用方法来识别小分子。适配体是能够特异性结合小分子的短链核酸。通过利用适配体提高识别技术的性能，在检测小分子时有可能实现高选择性和灵敏度。近年来，已开发出多种适配体靶标识别技术以提高检测小分子的能力。本综述重点关注检测平台的原理，对小分子与适配体之间的偶联方法进行分类，总结基于适配体的偶联识别技术在食品小分子检测方面的进展，并力求在即时诊断领域提供新兴的强大工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/1d5af674076a/foods-13-01749-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/bd171c3f6902/foods-13-01749-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/7106140d01ee/foods-13-01749-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/12f2c3025e25/foods-13-01749-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/31fa43efa75f/foods-13-01749-g008a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/010bba3a6f05/foods-13-01749-g009a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/50376c57155b/foods-13-01749-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/b4c734f8a013/foods-13-01749-g011a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/309bf26696a8/foods-13-01749-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/31e122890857/foods-13-01749-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/ef18a9d93e2d/foods-13-01749-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/1d5af674076a/foods-13-01749-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/bd171c3f6902/foods-13-01749-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/7106140d01ee/foods-13-01749-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/12f2c3025e25/foods-13-01749-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/31fa43efa75f/foods-13-01749-g008a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/010bba3a6f05/foods-13-01749-g009a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/50376c57155b/foods-13-01749-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/b4c734f8a013/foods-13-01749-g011a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/309bf26696a8/foods-13-01749-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/31e122890857/foods-13-01749-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/ef18a9d93e2d/foods-13-01749-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a379/11172347/1d5af674076a/foods-13-01749-g005a.jpg

相似文献

1
Advances in Aptamer-Based Conjugate Recognition Techniques for the Detection of Small Molecules in Food.基于适配体的共轭识别技术在食品中小分子检测方面的进展。
Foods. 2024 Jun 2;13(11):1749. doi: 10.3390/foods13111749.
2
"Fitting" makes "sensing" simple: label-free detection strategies based on nucleic acid aptamers.“适配”使“传感”变得简单：基于核酸适体的无标记检测策略。
Acc Chem Res. 2013 Feb 19;46(2):203-13. doi: 10.1021/ar300011g. Epub 2012 Dec 6.
3
Recent Advances in Biological Applications of Aptamer-Based Fluorescent Biosensors.基于适体的荧光生物传感器在生物应用中的最新进展。
Molecules. 2023 Oct 29;28(21):7327. doi: 10.3390/molecules28217327.
4
Using Exonucleases for Aptamer Characterization, Engineering, and Sensing.利用外切核酸酶进行适体表征、工程改造和传感。
Acc Chem Res. 2023 Jul 4;56(13):1731-1743. doi: 10.1021/acs.accounts.3c00113. Epub 2023 Jun 14.
5
Development and Application of Aptamer-Based Surface-Enhanced Raman Spectroscopy Sensors in Quantitative Analysis and Biotherapy.基于适体的表面增强拉曼光谱传感器在定量分析和生物治疗中的发展与应用。
Sensors (Basel). 2019 Sep 3;19(17):3806. doi: 10.3390/s19173806.
6
Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices.基于适配体的荧光生物传感器用于快速灵敏检测食品基质中的过敏原。
Foods. 2021 Oct 27;10(11):2598. doi: 10.3390/foods10112598.
7
Recent Advances in Biomolecular Detection Based on Aptamers and Nanoparticles.基于适体和纳米粒子的生物分子检测的最新进展。
Biosensors (Basel). 2023 Apr 13;13(4):474. doi: 10.3390/bios13040474.
8
Application of Electrochemical Aptasensors toward Clinical Diagnostics, Food, and Environmental Monitoring: Review.电化学适体传感器在临床诊断、食品和环境监测中的应用：综述。
Sensors (Basel). 2019 Dec 10;19(24):5435. doi: 10.3390/s19245435.
9
Graphene/aptamer probes for small molecule detection: from in vitro test to in situ imaging.石墨烯/适体探针用于小分子检测：从体外试验到原位成像。
Mikrochim Acta. 2020 Feb 19;187(3):179. doi: 10.1007/s00604-020-4128-8.
10
Aptamers and Aptasensors for Highly Specific Recognition and Sensitive Detection of Marine Biotoxins: Recent Advances and Perspectives.适体与适体传感器在海洋生物毒素的高特异性识别和灵敏检测中的应用：最新进展与展望。
Toxins (Basel). 2018 Oct 25;10(11):427. doi: 10.3390/toxins10110427.

本文引用的文献

1
Integration of a hamper pad on test strips for improved sensitivity of carbendazim detection.在测试条上集成一个收集垫，以提高多菌灵检测的灵敏度。
Talanta. 2024 Jun 1;273:125911. doi: 10.1016/j.talanta.2024.125911. Epub 2024 Mar 13.
2
Highly sensitive and label free on-site monitoring immunosensor for detection of Aflatoxin B from real samples.高灵敏度、无需标记的现场监测免疫传感器，用于检测真实样本中的黄曲霉毒素 B。
Anal Biochem. 2024 Jun;689:115493. doi: 10.1016/j.ab.2024.115493. Epub 2024 Feb 23.
3
Quantum Dot-based Bio-conjugates as an Emerging Bioimaging Tool for Cancer Theranostic- A Review.
基于量子点的生物缀合物作为癌症治疗诊断的新兴生物成像工具——综述
Curr Drug Targets. 2024;25(4):241-260. doi: 10.2174/0113894501283669240123105250.
4
A Label-Free Aptasensor for Turn-On Fluorescent Detection of Aflatoxin B1 Based on an Aggregation-Induced-Emission-Active Probe and Single-Walled Carbon Nanohorns.基于聚集诱导发光活性探针和单壁碳纳米角的用于开启式荧光检测黄曲霉毒素B1的无标记适体传感器。
Foods. 2023 Dec 1;12(23):4332. doi: 10.3390/foods12234332.
5
A signal-on photoelectrochemical aptasensor based on ferrocene labeled triple helix DNA molecular switch for detection of antibiotic amoxicillin.基于三螺旋 DNA 分子开关标记的钌联吡啶的信号放大光电化学适体传感器用于检测抗生素阿莫西林。
Food Chem. 2024 May 30;441:138333. doi: 10.1016/j.foodchem.2023.138333. Epub 2024 Jan 3.
6
Facile Synthesis of Platinum Nanoparticle-Embedded Reduced Graphene Oxide for the Detection of Carbendazim.用于检测多菌灵的铂纳米粒子嵌入还原氧化石墨烯的简便合成法
Materials (Basel). 2023 Dec 13;16(24):7622. doi: 10.3390/ma16247622.
7
An Aptamer-Based Lateral Flow Biosensor for Low-Cost, Rapid and Instrument-Free Detection of Ochratoxin A in Food Samples.基于适体的侧向流生物传感器用于低成本、快速和无仪器检测食品样品中的赭曲霉毒素 A。
Molecules. 2023 Dec 17;28(24):8135. doi: 10.3390/molecules28248135.
8
Screening of broad-spectrum aptamer and development of electrochemical aptasensor for simultaneous detection of penicillin antibiotics in milk.广谱适体的筛选及电化学适体传感器的构建用于牛奶中青霉素类抗生素的同时检测
Talanta. 2024 Mar 1;269:125508. doi: 10.1016/j.talanta.2023.125508. Epub 2023 Dec 5.
9
A versatile CuCo@PDA nanozyme-based aptamer-mediated lateral flow assay for highly sensitive, on-site and dual-readout detection of Aflatoxin B1.一种基于多功能 CuCo@PDA 纳米酶的适体介导侧向流分析方法，用于高灵敏、现场和双读数检测黄曲霉毒素 B1。
J Hazard Mater. 2024 Mar 5;465:133178. doi: 10.1016/j.jhazmat.2023.133178. Epub 2023 Dec 5.
10
Target-Induced Electrochemical Sensor Based on Foldable Aptamer and MoS@MWCNTs-PEI for Enhanced Detection of AFB1 in Peanuts.基于折叠适体和 MoS@MWCNTs-PEI 的靶向电化学传感器用于增强花生中 AFB1 的检测。
Langmuir. 2023 Nov 21;39(46):16422-16431. doi: 10.1021/acs.langmuir.3c02216. Epub 2023 Nov 7.