Department of Biochemistry and Molecular Biology and Agrifood Campus of International Excellence (ceiA3), University of Córdoba, Severo Ochoa Building, Rabanales Campus, 14071 Córdoba, Spain.
Department of Chemistry and Materials Science, Faculty of Experimental Science and Agrifood Campus of International Excellence (ceiA3), University of Huelva, El Carmen Campus, 21007 Huelva, Spain.
Sci Total Environ. 2017 Apr 15;584-585:813-827. doi: 10.1016/j.scitotenv.2017.01.125. Epub 2017 Jan 31.
A 2D-DIGE/MS approach was used to assess protein abundance differences in the red swamp crayfish Procambarus clarkii from polluted aquatic ecosystems of Doñana National Park and surrounding areas with different pollution loads. Procambarus clarkii accumulated metals in the digestive glands and gills reflecting sediment concentrations. We first stated that, probably related to elements accumulation, pollution increased oxidative damage in P. clarkii tissues, as shown by the thiol oxidation status of proteins and MDA levels. In these animals, the altered redox status might be responsible for the deregulated abundance of proteins involved in cellular responses to oxidative stress including protein folding, mitochondrial imbalance and inflammatory processes. Interestingly, polluted P. clarkii crayfish also displayed a metabolic shift to enhanced aerobic glycolysis, most likely aimed at generating ATP and reduction equivalents in an oxidative stress situation that alters mitochondrial integrity. The deregulated proteins define the physiological processes affected by pollutants in DNP and its surrounding areas and may help us to unravel the molecular mechanisms underlying the toxicity of environmental pollutants. In addition, these proteins might be used as exposure biomarkers in environmental risk assessment. The results obtained might be extrapolated to many other locations all over the world and have the added value of providing information about the molecular responses of this environmentally and economically interesting animal.
Metal content in digestive gland and gills of P. clarkii crayfish reflects their contents in sediments at sites of Doñana National Park and its surroundings. Accumulation of essential and toxic transition metals is paralleled by clear signs of oxidative stress to lipids and proteins and by significant deregulation of many proteins involved in protein folding, mitochondrial respiratory imbalance and inflammatory response. These results indicate that P. clarkii is an excellent bioindicator to be used in aquatic ecosystems quality monitoring. Additionally, results evidence that the anthropogenic activities carried out around Doñana National Park represent an extremely serious threat to this unique Biosphere Reserve and pose a risk to the environment and their inhabitants health. The identified deregulated proteins provide information about the metabolic pathways and/or physiological processes affected by pollutant-elicited oxidative stress, may also be useful as biomarkers of environmental pollution and have the added value of providing information about the molecular responses of this environmentally and economically interesting animal.
采用 2D-DIGE/MS 方法评估来自多纳纳国家公园受污染水生生态系统和周围不同污染负荷地区的红沼泽小龙虾 Procambarus clarkii 中蛋白质丰度的差异。Procambarus clarkii 在其消化腺和鳃中积累了金属，反映了沉积物中的浓度。我们首先指出，可能与元素积累有关，污染增加了 P. clarkii 组织中的氧化损伤，如蛋白质的硫醇氧化状态和 MDA 水平所示。在这些动物中，改变的氧化还原状态可能是导致参与细胞对氧化应激反应的蛋白质丰度失调的原因，包括蛋白质折叠、线粒体失衡和炎症过程。有趣的是，受污染的 P. clarkii 小龙虾还表现出代谢向增强有氧糖酵解的转变，这很可能是为了在改变线粒体完整性的氧化应激情况下产生 ATP 和还原当量。失调的蛋白质定义了污染物在 DNP 及其周围地区影响的生理过程，并可能有助于我们揭示环境污染物毒性的分子机制。此外，这些蛋白质可用作环境风险评估中的暴露生物标志物。获得的结果可以推断到世界上许多其他地方，并提供有关这种具有环境和经济意义的动物的分子反应的信息，这是其附加值。
P. clarkii 小龙虾消化腺和鳃中的金属含量反映了它们在多纳纳国家公园及其周围地区沉积物中的含量。必需和毒性过渡金属的积累伴随着脂质和蛋白质明显的氧化应激迹象，以及许多参与蛋白质折叠、线粒体呼吸失衡和炎症反应的蛋白质的显著失调。这些结果表明，P. clarkii 是水生生态系统质量监测的极好生物标志物。此外，结果表明，多纳纳国家公园周围开展的人类活动对这个独特的生物圈保护区构成了极其严重的威胁，并对环境及其居民的健康构成了威胁。鉴定出的失调蛋白质提供了有关受污染物诱导的氧化应激影响的代谢途径和/或生理过程的信息，也可用作环境污染的生物标志物，并具有提供有关这种具有环境和经济意义的动物的分子反应的信息的附加值。
