Shokri H, Sharifzadeh A
Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Imam Khomeini Street, 24th Aftab, Amol, Iran.
Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
J Mycol Med. 2017 Jun;27(2):159-165. doi: 10.1016/j.mycmed.2017.01.004. Epub 2017 Jan 31.
Honey is well known to possess a broad spectrum of activity against medically important organisms. The purpose of this study was to assess the antifungal activity of different honeys against 40 fluconazole (FLU) resistant Candida species, including Candida albicans (C. albicans), Candida glabrata, Candida krusei and Candida tropicalis.
Three honey samples were collected from northern (Mazandaran, A), southern (Hormozgan, B) and central (Lorestan, C) regions of Iran. A microdilution technique based on the CLSI, M27-A2 protocol was employed to compare the susceptibility of honeys "A", "B" and "C" against different pathogenic Candida isolates.
The results showed that different Candida isolates were resistant to FLU, ranging from 64μg/mL to 512μg/mL. All of the honeys tested had antifungal activities against FLU-resistant Candida species, ranging from 20% to 56.25% (v/v) and 25% to 56.25% (v/v) for minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs), respectively. Honey "A" (MIC: 31.59%, v/v) showed higher anti-Candida activity than honey "B" (MIC: 35.99%, v/v) and honey "C" (MIC: 39.2%, v/v). No statistically significant differences were observed among the mean MIC values of the honey samples (P>0.05). The order of overall susceptibility of Candida species to honey samples were; C. krusei>C. glabrata>C. tropicalis>C. albicans (P>0.05). In addition, the mean MICs of Candida strains isolated from the nail, vagina and oral cavity were 33.68%, 36.44% and 39.89%, respectively, and were not significantly different (P>0.05).
Overall, varying susceptibilities to the anti-Candida properties of different honeys were observed with four FLU-resistant species of Candida. Further research is needed to assess the efficacy of honey as an inhibitor of candidal growth in clinical trials.
蜂蜜以对医学上重要的微生物具有广泛的活性而闻名。本研究的目的是评估不同蜂蜜对40种耐氟康唑（FLU）的念珠菌属的抗真菌活性，包括白色念珠菌、光滑念珠菌、克柔念珠菌和热带念珠菌。
从伊朗北部（马赞德兰，A）、南部（霍尔木兹甘，B）和中部（洛雷斯坦，C）地区采集了三个蜂蜜样本。采用基于CLSI M27 - A2协议的微量稀释技术，比较蜂蜜“A”、“B”和“C”对不同致病性念珠菌分离株的敏感性。
结果表明，不同的念珠菌分离株对氟康唑的耐药性在64μg/mL至512μg/mL之间。所有测试的蜂蜜对耐氟康唑的念珠菌属均具有抗真菌活性，最小抑菌浓度（MIC）范围为20%至56.25%（v/v），最小杀菌浓度（MFC）范围为25%至56.25%（v/v）。蜂蜜“A”（MIC：31.59%，v/v）显示出比蜂蜜“B”（MIC：35.99%，v/v）和蜂蜜“C”（MIC：39.2%，v/v）更高的抗念珠菌活性。蜂蜜样本的平均MIC值之间未观察到统计学上的显著差异（P>0.05）。念珠菌属对蜂蜜样本的总体敏感性顺序为：克柔念珠菌>光滑念珠菌>热带念珠菌>白色念珠菌（P>0.05）。此外，从指甲、阴道和口腔分离的念珠菌菌株的平均MIC分别为33.68%、36.44%和39.89%，且无显著差异（P>0.05）。
总体而言，观察到四种耐氟康唑的念珠菌对不同蜂蜜的抗念珠菌特性的敏感性各不相同。需要进一步研究以评估蜂蜜在临床试验中作为念珠菌生长抑制剂的疗效。
