Movahedi M, Tavakol M, Rahmani F, Amirzargar A A, Bidoki A Z, Heidari K, Gharagozlou M, Aghamohammadi A, Nabavi M, Soltani S, Rezaei N
Department of Pediatrics, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
Department of Allergy and Clinical Immunology, Shahid Bahonar Hospital, Alborz University of Medical Sciences, Karaj, Iran.
Allergol Immunopathol (Madr). 2017 Jul-Aug;45(4):333-338. doi: 10.1016/j.aller.2016.10.009. Epub 2017 Feb 1.
A clear picture of interaction of Th1/Th2 cytokines in pathogenesis of chronic spontaneous urticaria (CSU), remains elusive. Impaired IFN-γ production and decreased levels of IL-2 have been reported. The aim of this study was to evaluate the association of Th1 cytokines; IL-2, IL-12 and IFN-γ polymorphisms with CSU.
90 patients with CSU and 140 age-sex matched subjects were included in this study. DNA samples were evaluated through PCR-SSP assay in order to detect single nucleotide polymorphisms of IL-12 (A/C -1188) or (rs3212227), IFN-γ (A/T UTR5644) or (rs2069717) and IL-2 (G/T -330 and G/T +166) or (rs2069762 and rs2069763).
G allele at -330 at promoter region of IL-2 gene was overrepresented in CSU. Heterozygotes (GT) at this locus and heterozygotes at +166 of IL-2 gene (GT) were more prevalent in CSU group. Additionally, the haplotype GT for loci -330 and +166 of IL-2 gene was powerfully associated with CSU (OR (95%CI)=57.29 (8.43-112.7)).
SNP at position -330 and +166 of IL-2 gene are differently expressed in CSU. The haplotype GT of IL-2 at -330 and +166 might confer vulnerability to a number of immunological disorders in Iranian region.
Th1/Th2细胞因子在慢性自发性荨麻疹（CSU）发病机制中的相互作用尚不清楚。已有报道称干扰素-γ（IFN-γ）产生受损和白细胞介素-2（IL-2）水平降低。本研究旨在评估Th1细胞因子IL-2、IL-12和IFN-γ基因多态性与CSU的相关性。
本研究纳入了90例CSU患者和140例年龄及性别匹配的受试者。通过聚合酶链反应-序列特异性引物分析（PCR-SSP）检测DNA样本，以检测IL-12（A/C -1188）或（rs3212227）、IFN-γ（A/T UTR5644）或（rs2069717）以及IL-2（G/T -330和G/T +166）或（rs2069762和rs2069763）的单核苷酸多态性。
IL-2基因启动子区-330位点的G等位基因在CSU中出现频率较高。该位点的杂合子（GT）以及IL-2基因+166位点的杂合子（GT）在CSU组中更为常见。此外，IL-2基因-330和+166位点的单倍型GT与CSU密切相关（比值比（95%置信区间）=57.29（8.43-112.7））。
IL-2基因-330和+166位点的单核苷酸多态性在CSU中的表达存在差异。IL-2基因-330和+166位点的单倍型GT可能使伊朗地区人群易患多种免疫性疾病。
