Andersen Karina R, Harsløf Laurine B S, Schnurr Theresia M, Hansen Torben, Hellgren Lars I, Michaelsen Kim F, Lauritzen Lotte
1Department of Nutrition, Exercise & Sports,University of Copenhagen,1958 Frederiksberg,Denmark.
2Department of Hematology, Rigshospitalet,Copenhagen Biocenter,2100 Copenhagen,Denmark.
Br J Nutr. 2017 Jan;117(2):278-286. doi: 10.1017/S0007114516004645. Epub 2017 Feb 6.
DHA from diet or endogenous synthesis has been proposed to affect infant development, however, results are inconclusive. In this study, we aim to verify previously observed fatty acid desaturase gene cluster (FADS) SNP-specific associations with erythrocyte DHA status in 9-month-old children and sex-specific association with developmental outcomes. The study was performed in 166 children (55 % boys) of obese mothers. Erythrocyte fatty acid composition was analysed in blood-samples obtained at 9 months of age, and developmental outcomes assessed by the Ages and Stages Questionnaire at 3 years. Erythrocyte DHA level ranged from 4·4 to 9·9 % of fatty acids, but did not show any association with FADS SNP or other potential determinants. Regression analysis showed associations between erythrocyte DHA and scores for personal-social skills (β 1·8 (95 % CI 0·3, 3·3), P=0·019) and problem solving (β 3·4 (95 % CI 1·2, 5·6), P=0·003). A tendency was observed for an association in opposite direction between minor alleles (G-variant) of rs1535 and rs174575 and personal-social skills (P=0·062 and 0·068, respectively), which became significant when the SNP were combined based on their previously observed effect on erythrocyte DHA at 9 months of age (β 2·6 (95 % CI 0·01, 5·1), P=0·011). Sex-SNP interaction was indicated for rs174575 genotype on fine motor scores (P=0·016), due to higher scores among minor allele carrying girls (P=0·043), whereas no effect was seen among boys. In conclusion, DHA-increasing FADS SNP and erythrocyte DHA status were consistently associated with improved personal-social skills in this small cohort of children of obese mothers irrespective of sex, but the sample was too small to verify potential sex-specific effects.
饮食来源或内源性合成的DHA被认为会影响婴儿发育，然而，结果尚无定论。在本研究中，我们旨在验证先前观察到的脂肪酸去饱和酶基因簇（FADS）单核苷酸多态性（SNP）与9个月大儿童红细胞DHA水平的特异性关联，以及与发育结局的性别特异性关联。该研究在166名肥胖母亲的子女（55%为男孩）中进行。分析了9个月大时采集的血样中的红细胞脂肪酸组成，并在3岁时通过年龄与发育进程问卷评估发育结局。红细胞DHA水平占脂肪酸的4.4%至9.9%，但与FADS SNP或其他潜在决定因素无任何关联。回归分析显示，红细胞DHA与个人社交技能得分（β 1.8（95%CI 0.3，3.3），P = 0.019）和解决问题能力得分（β 3.4（95%CI 1.2，5.6），P = 0.003）之间存在关联。观察到rs1535和rs174575的次要等位基因（G变异）与个人社交技能之间存在相反方向的关联趋势（分别为P = 0.062和0.068），当根据它们先前对9个月大儿童红细胞DHA的影响将SNP合并时，这种关联变得显著（β 2.6（95%CI 0.01，5.1），P = 0.011）。rs174575基因型在精细运动得分上存在性别-SNP相互作用（P = 0.016），因为携带次要等位基因的女孩得分较高（P = 0.043），而男孩中未观察到影响。总之，在这一小群肥胖母亲的子女中，增加DHA的FADS SNP和红细胞DHA水平无论性别均与个人社交技能的改善持续相关，但样本量太小，无法验证潜在的性别特异性影响。
