Yuan He, Xu Pengjun, Yang Xianming, Graham Robert I, Wilson Kenneth, Wu Kongming
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, PR China.
J Invertebr Pathol. 2017 Mar;144:65-73. doi: 10.1016/j.jip.2017.01.011. Epub 2017 Feb 3.
The cotton bollworm, Helicoverpa armigera, is one of the most important agricultural pests of many economic crops worldwide. Herein, we found a novel single-strand RNA virus by RNA-Seq and Polymerase Chain Reaction (PCR) method in H. armigera named Helicoverpa armigera iflavirus (HaIV), which possessed a genome with 10,017 nucleotides in length and contained a single large open reading frame (ORF) encoding a putative polyprotein of 3021 amino acids with a predicted molecular mass of 344.16kDa and a theoretical isoelectric point (pI) of 6.45. The deduced amino acid sequence showed highest similarity (61.0%) with the protein of Lymantria dispar Iflavirus 1. Phylogenetic analysis with putative RdRp amino acid sequences indicated that the virus clustered with members of the genus Iflavirus. The virus was mainly distributed in the fat body of its host and was found to be capable of both horizontal and vertical transmission. The efficiency of perorally horizontal transmission was dose dependent (100% infection rate with a viral dose of 10copies/μl) while vertical transmission efficiency was found to be relatively low (<28.57%). These results suggest that we have found a novel member of genus Iflavirus in H. armigera.
棉铃虫（Helicoverpa armigera）是全球许多经济作物最重要的农业害虫之一。在此，我们通过RNA测序和聚合酶链反应（PCR）方法在棉铃虫中发现了一种新型单链RNA病毒，命名为棉铃虫伊弗病毒（HaIV），其基因组长度为10,017个核苷酸，包含一个单一的大开放阅读框（ORF），编码一个推定的多蛋白，由3021个氨基酸组成，预测分子量为344.16 kDa，理论等电点（pI）为6.45。推导的氨基酸序列与舞毒蛾伊弗病毒1的蛋白质具有最高的相似性（61.0%）。用推定的RNA依赖的RNA聚合酶（RdRp）氨基酸序列进行系统发育分析表明，该病毒与伊弗病毒属的成员聚集在一起。该病毒主要分布在其宿主的脂肪体中，并且发现它能够进行水平和垂直传播。经口水平传播的效率取决于剂量（病毒剂量为10拷贝/微升时感染率为100%），而垂直传播效率相对较低（<28.57%）。这些结果表明，我们在棉铃虫中发现了伊弗病毒属的一个新成员。
