Lin Hong, Song Weitao, Ma Dongmei, Yang Chi, Yao Yanfang, Liu Renyi, Hao Ling, Wu Dandan, Wang Shihua, Jiang Jimou, Xiong Jun, Ma Rui, Xiao Jiajing, Zhuang Zhenhong
Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, Proteomic Research Center, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Insects. 2024 Aug 19;15(8):625. doi: 10.3390/insects15080625.
is one of the common pests of fruit trees, but the research on the pathogenic microorganisms it may carry is very limited. Therefore, it is essential to reveal the pathogenic microbes it carries and their potential hazards. This study found a new virus from the transcriptome of , which was first reported in and named PyCaV ( associated virus). RACE and bioinformatics assay revealed that the full length of PyCaV is 10,855 bp with the polyA tail, containing a single open-reading frame (ORF) encoding a polyprotein consisting of 3171 amino acid (aa). The virus has a typical iflavirus structure, including two domains, an RNA helicase domain (HEL), a 3C cysteine protease domain (Pro), and an RNA-dependent RNA polymerase domain (RdRp). Further phylogenetic analysis revealed that this virus belongs to family and sequence alignments analysis suggested PyCaV is a new member in an unassigned genus of family . Further in-depth analysis of the virus infection showed that PyCaV is distributed throughout the whole , including its head, chest, and abdomen, but more PyCaV was identified in the chest. The distribution of PyCaV in different parts of was further explored, which showed that more PyCaV was detected in its piercing-sucking mouthparts and chest viscera. Statistical analysis showed that the PyCaV infection was affected by time and location.
是果树的常见害虫之一，但对其可能携带的致病微生物的研究非常有限。因此，揭示其携带的致病微生物及其潜在危害至关重要。本研究从的转录组中发现了一种新病毒，该病毒于首次报道并命名为PyCaV（相关病毒）。RACE和生物信息学分析表明，PyCaV全长10855bp，带有polyA尾，包含一个单一的开放阅读框（ORF），编码一个由3171个氨基酸（aa）组成的多聚蛋白。该病毒具有典型的黄病毒结构，包括两个结构域、一个RNA解旋酶结构域（HEL）、一个3C半胱氨酸蛋白酶结构域（Pro）和一个RNA依赖性RNA聚合酶结构域（RdRp）。进一步的系统发育分析表明，该病毒属于科，序列比对分析表明PyCaV是科一个未分类属的新成员。对病毒感染的进一步深入分析表明，PyCaV分布于整个，包括其头部、胸部和腹部，但在胸部发现的PyCaV更多。进一步探索了PyCaV在不同部位的分布，结果表明在其刺吸式口器和胸内脏器中检测到更多的PyCaV。统计分析表明，PyCaV感染受时间和地点的影响。 （注：原文中部分关键主体未明确写出，翻译可能存在信息不完整的情况）
