Kaneko Toshiro, Sasaki Shota, Takashima Keisuke, Kanzaki Makoto
Department of Electronic Engineering, Tohoku University, 6-6-05 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
Department of Biomedical Engineering, Tohoku University, 6-6-05 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
J Clin Biochem Nutr. 2017 Jan;60(1):3-11. doi: 10.3164/jcbn.16-73. Epub 2016 Dec 17.
Gas-liquid interfacial atmospheric-pressure plasma jets (GLI-APPJ) are used medically for plasma-induced cell-membrane permeabilization. In an attempt to identify the dominant factors induced by GLI-APPJ responsible for enhancing cell-membrane permeability, the concentration and distribution of plasma-produced reactive species in the gas and liquid phase regions are measured. These reactive species are classified in terms of their life-span: long-lived (e.g., HO), short-lived (e.g., O), and extremely-short-lived (e.g., OH). The concentration of plasma-produced OH in the liquid phase region decreases with an increase in solution thickness (<1 mm), and plasma-induced cell-membrane permeabilization is found to decay markedly as the thickness of the solution increases. Furthermore, the horizontally center-localized distribution of OH, resulting from the center-peaked distribution of OH in the gas phase region, corresponds with the distribution of the permeabilized cells upon APPJ irradiation, whereas the overall plasma-produced oxidizing species such as HO in solution exhibit a doughnut-shaped horizontal distribution. These results suggest that OH is likely one of the dominant factors responsible for plasma-induced cell-membrane permeabilization.
气液界面大气压等离子体射流（GLI - APPJ）在医学上用于等离子体诱导的细胞膜通透性增强。为了确定GLI - APPJ诱导的导致细胞膜通透性增强的主要因素，对气相和液相区域中等离子体产生的活性物种的浓度和分布进行了测量。这些活性物种根据其寿命进行分类：长寿命（例如，HO）、短寿命（例如，O）和极短寿命（例如，OH）。液相区域中等离子体产生的OH浓度随着溶液厚度（<1毫米）的增加而降低，并且发现随着溶液厚度的增加，等离子体诱导的细胞膜通透性显著衰减。此外，由于气相区域中OH呈中心峰值分布而导致的OH水平中心局部化分布，与APPJ照射后通透细胞的分布相对应，而溶液中整体等离子体产生的氧化物种如HO呈现出甜甜圈形状的水平分布。这些结果表明，OH可能是导致等离子体诱导细胞膜通透性增强的主要因素之一。
