Deeley Kathleen, Noel Jacqueline, Vieira Alexandre R
Clin Lab. 2016 Sep 1;62(9):1809-1813. doi: 10.7754/Clin.Lab.2016.160207.
Obtaining DNA samples through saliva samples is becoming more common, and more companies are responding to this demand with saliva collection/DNA extraction kits.
The University of Pittsburgh's School of Dental Medicine maintains a DNA bank, which our lab helps direct. While we have always used Oragene (DNAGenotek, Kanata, Canada) kits to collect samples in the past, we recently compared 5 alternative kits/methods in an effort to reduce costs while maintaining quality. The kits/ methods were: Norgen (Norgen Biotek Corp., Thorold, Canada), Stratec (Stratec Biomedical, Birkenfeld, Germany), DNAgard (Biomatrica, San Diego, CA, USA), Oasis (Oasis Diagnostics, Vancouver, WA, USA), and an inhouse protocol for DNA extraction from whole saliva. We compared 7 protocols for extracting DNA from saliva using 5 commercially available kits. We primarily looked at total DNA yield, but also considered cost, ease of sample collection, and complexity of extraction protocol.
When compared to the Oragene kits, only Norgen and Startec had comparable DNA yields (30 µg or more). Oasis was the easiest to use in terms of sample collection.
When compared to our whole saliva DNA extraction protocol, all kits had higher yields, shorter extraction time, and easier protocols.
通过唾液样本获取DNA样本正变得越来越普遍，越来越多的公司用唾液采集/DNA提取试剂盒来满足这一需求。
匹兹堡大学牙医学院设有一个DNA库，我们实验室协助管理该DNA库。过去我们一直使用Oragene（加拿大渥太华DNAGenotek公司）试剂盒来采集样本，最近我们比较了5种替代试剂盒/方法，以便在保持质量的同时降低成本。这些试剂盒/方法分别是：Norgen（加拿大索罗尔德Norgen Biotek公司）、Stratec（德国比肯费尔德Stratec Biomedical公司）、DNAgard（美国加利福尼亚州圣地亚哥Biomatrica公司）、Oasis（美国华盛顿州温哥华Oasis Diagnostics公司），以及一种从全唾液中提取DNA的内部方案。我们使用5种市售试剂盒比较了7种从唾液中提取DNA的方案。我们主要关注总DNA产量，但也考虑了成本、样本采集的难易程度以及提取方案的复杂性。
与Oragene试剂盒相比，只有Norgen和Startec的DNA产量相当（30微克或更多）。就样本采集而言，Oasis最易于使用。
与我们的全唾液DNA提取方案相比，所有试剂盒的产量更高、提取时间更短且方案更简便。
