Shimabukuro T, Yamamoto M, Mitsui H, Yamamoto N, Sakatoku J, Harada H, Sacho T
Department of Urology, Yamaguchi University School of Medicine.
Hinyokika Kiyo. 1989 Aug;35(8):1285-90.
With the aim to apply a rapid analytic method of cell kinetics using in vitro BrdU labelling and anti-BrdU monoclonal antibody to the clinical fields, we investigated the cell kinetics of 20 urogenital malignant tumors. The DNA synthesizing cells which take up BrdU in its nuclei can be detected so clearly, since the labelling index (LI) is easily identified. The correlations between LI and age and with histological grade and stage of 14 transitional cell cancers of urinary tract were investigated. There was no correlation between LI and age. The LI of high grade groups (G2 & G3) were significantly higher than that of the low grade group (G1) (p less than 0.05). Also between LI of pT1 group and pT3 group, there was a statistical significance (p less than 0.05) and generally LI tended to increase according to stage progression. Therefore, the cytokinetic data obtained from the before mentioned method can be utilized as a useful marker for measuring malignant potentials of each tumor.
为了将使用体外BrdU标记和抗BrdU单克隆抗体的细胞动力学快速分析方法应用于临床领域，我们研究了20例泌尿生殖系统恶性肿瘤的细胞动力学。由于易于识别标记指数（LI），因此可以清晰地检测到细胞核中摄取BrdU的DNA合成细胞。研究了14例泌尿系统移行细胞癌的LI与年龄、组织学分级和分期之间的相关性。LI与年龄之间无相关性。高级别组（G2和G3）的LI显著高于低级别组（G1）（p<0.05）。此外，pT1组和pT3组的LI之间存在统计学意义（p<0.05），并且一般来说LI倾向于随着分期进展而增加。因此，从上述方法获得的细胞动力学数据可作为衡量各肿瘤恶性潜能的有用标志物。
