Ren Zhenxin, Liu Ruiyuan, Gu Wenting, Dong Xicun
Department of Space Radiobiology, Key Laboratory of Heavy Ion Radiation Biology and Medicine, Institute of Modern Physics, Chinese Academy of Sciences, China.
Department of Biophysics, Institute of Modern Physics, Chinese Academy of Sciences, China.
Plant Sci. 2017 Mar;256:103-111. doi: 10.1016/j.plantsci.2016.12.008. Epub 2016 Dec 29.
ARF2 as apleiotropic developmental regulator has been reported in Arabidopsis thaliana and tomato (Solanum lycopersicum). The present study showed SlARF2 transcripts in all tomato plant tissues but with higher accumulation in flowers. During bud-anthesis stages, SlARF2 transcripts showed a dynamic expression pattern in sepal, stamen, ovary and petal. Hormone treatment analysis suggested that SlARF2 transcript accumulation was positively regulated by auxin and gibberellic acid, and negatively regulated by ethylene in tomato seedlings. Phenotypes and molecular analyses of SlARF2-upregulated transgenic tomato indicated that SlARF2 regulated tomato lateral root formation and flower organ senescence may be partially mediated by regulating the gene expression of auxin and ethylene response factors. The data enlarges the functional characterization of SlARF2 in tomato, and broadens our understanding of auxin signaling in regulating plant growth and development.
ARF2作为一种多效性发育调节因子，已在拟南芥和番茄（Solanum lycopersicum）中被报道。本研究表明，SlARF2转录本在番茄植株的所有组织中均有表达，但在花中的积累量更高。在花蕾期到开花期阶段，SlARF2转录本在萼片、雄蕊、子房和花瓣中呈现动态表达模式。激素处理分析表明，在番茄幼苗中，SlARF2转录本的积累受到生长素和赤霉素的正向调节，而受到乙烯的负向调节。对SlARF2上调的转基因番茄的表型和分子分析表明，SlARF2调节番茄侧根形成和花器官衰老可能部分是通过调节生长素和乙烯反应因子的基因表达来介导的。这些数据扩展了SlARF2在番茄中的功能特性，并拓宽了我们对生长素信号在调节植物生长发育中的理解。
