Crist Angela M, Young Chandler, Meadows Stryder M
Department of Cell and Molecular Biology, Tulane University, USA.
Department of Cell and Molecular Biology, Tulane University, USA.
Gene Expr Patterns. 2017 Jan;23-24:22-31. doi: 10.1016/j.gep.2017.01.002. Epub 2017 Feb 3.
The murine retina has become an ideal model to study blood vessel formation. Blood vessels in the retina undergo various processes, including remodeling and differentiation, to form a stereotypical network that consists of precisely patterned arteries and veins. This model presents a powerful tool for understanding many different aspects of angiogenesis including artery and vein (AV) cell fate acquisition and differentiation. However, characterization of AV differentiation has been largely unexplored in the mouse retinal model. In this study, we describe the expression of previously established AV markers and assess arteriovenous acquisition and identity in the murine neonatal retina. Using in situ hybridization and immunofluorescent antibody staining techniques, we analyzed numerous AV differentiation markers such as EphB4-EphrinB2 and members of the Notch pathway. We find that at postnatal day 3 (P3), when blood vessels are beginning to populate the retina, AV identity is not immediately established. However, by P5 expression of many molecular identifiers of arteries and veins become restricted to their respective vessel types. This molecular distinction is more obvious at P7 and remains unchanged through P9. Overall, these studies indicate that, similar to the embryo, acquisition of AV identity occurs in a step-wise process and is largely established by P7 during retina development.
小鼠视网膜已成为研究血管形成的理想模型。视网膜中的血管经历各种过程，包括重塑和分化，以形成一个由精确排列的动脉和静脉组成的典型网络。该模型为理解血管生成的许多不同方面提供了一个强大的工具，包括动脉和静脉（AV）细胞命运的获得和分化。然而，在小鼠视网膜模型中，AV分化的特征在很大程度上尚未得到探索。在本研究中，我们描述了先前建立的AV标志物的表达，并评估了小鼠新生视网膜中动静脉的获得和特征。使用原位杂交和免疫荧光抗体染色技术，我们分析了许多AV分化标志物，如EphB4-EphrinB2和Notch信号通路的成员。我们发现，在出生后第3天（P3），当血管开始在视网膜中分布时，AV特征并未立即建立。然而，到P5时，许多动脉和静脉的分子标志物的表达开始局限于各自的血管类型。这种分子差异在P7时更加明显，并在P9时保持不变。总体而言，这些研究表明，与胚胎相似，AV特征的获得是一个逐步的过程，并且在视网膜发育过程中主要在P7时建立。
