Popova Anfisa, Tsvirkun Darya, Dolgov Oleg, Anokhin Konstantin, Alberts Jeffrey, Lagereva Evgeniia, Custaud Marc-Antoine, Gauquelin-Koch Guillemette, Vinogradova Olga, Andreev-Andrievskiy Alexander
Institute for Biomedical Problems, Russian Academy of Sciences, Khoroshevskoe Shosse, 76A, Moscow, 123007, Russia; Moscow State University, Biology Faculty, Leninskie Gory, 1/12, Moscow, 119234, Russia.
Research Center for Obstetrics, Gynecology and Parinatology, Ministry of Healthcare of the Russian Federation, Oparina Street, 4, Moscow, 117997, Russia; Laboratory of Integrated Neurovascular and Mitochondrial Biology (BNMI), UMR CNRS 6214, INSERM 1083, Angers, France.
J Pharmacol Toxicol Methods. 2017 May-Jun;85:29-37. doi: 10.1016/j.vascn.2017.02.002. Epub 2017 Feb 3.
Implantable telemetry enables continuous monitoring of physiological functions in freely moving animals and can greatly complement pharmacological research. Despite its miniaturization, a sensor/transmitter constitutes 5% or more of a mouse's bodyweight. The aim of the present study was to evaluate whether factors related to the presence of a probe/transmitter influence the ambulatory activity, strength, agility, or operant, motivated behaviors of this small rodent.
Adult male mice (C57BL/6N, 22-25g, 9-10weeks; implanted n=26, intact n=45) were evaluated during week-long tests, conducted three and eight weeks after surgical implantation of the PA-C10 blood pressure probe. An open field test, grip force measurement, Rotarod test were performed, followed by 7-day continuous monitoring of spontaneous wheel running activity and positively reinforced operant conditioning in an automated data collection system.
An implanted blood pressure transmitter did not affect behavior of mice in the open field test, on the Rotarod or their grip force, compared to unoperated controls. Voluntary wheel running distance was reduced three, but not eight weeks after implantation. Three weeks after the surgery, performance in the positively reinforced operant conditioning in operated mice was slightly decreased compared to intact animals, while retention and acquisition of a 2nd, reversal-learning task eight weeks after the surgery were unaffected.
We conclude that an implantable transmitter may have detectable effects in the first few weeks following implantation on some elements of mouse behavior. With sufficient recovery, mice perform comparably to unoperated controls in tests of strength, endurance, agility and learned operant behavior.
植入式遥测技术能够对自由活动动物的生理功能进行连续监测，可极大地辅助药理学研究。尽管其已实现小型化，但传感器/发射器仍占小鼠体重的5%或更多。本研究旨在评估与探针/发射器存在相关的因素是否会影响这种小型啮齿动物的自主活动、力量、敏捷性或操作性动机行为。
成年雄性小鼠（C57BL/6N，22 - 25克，9 - 10周龄；植入组n = 26，完整组n = 45）在植入PA - C10血压探针后的第3周和第8周进行为期一周的测试时接受评估。进行了旷场试验、握力测量、转棒试验，随后在自动数据收集系统中对自发转轮活动进行7天的连续监测以及对阳性强化操作性条件反射进行监测。
与未手术的对照组相比，植入的血压发射器并未影响小鼠在旷场试验、转棒试验中的行为或其握力。植入后3周时，自发转轮距离缩短，但8周时未缩短。手术后3周，手术小鼠在阳性强化操作性条件反射中的表现与完整动物相比略有下降，而手术后8周时第二个逆向学习任务的保持和习得未受影响。
我们得出结论，植入式发射器在植入后的最初几周可能会对小鼠行为的某些方面产生可检测到的影响。经过充分恢复后，小鼠在力量、耐力、敏捷性和习得的操作性行为测试中的表现与未手术的对照组相当。
