Kramer K, Voss H P, Grimbergen J A, Mills P A, Huetteman D, Zwiers L, Brockway B
Leiden/Amsterdam Center for Drug Research, Department of Pharmacochemistry, Faculty of Chemistry, Vrije Universiteit, Amsterdam, The Netherlands.
Lab Anim. 2000 Jul;34(3):272-80. doi: 10.1258/002367700780384663.
This paper describes for the first time the possibility for recording the systolic pressure (SP), diastolic pressure (DP), and the mean arterial pressure (MAP) as well as the heart rate (HR) and locomotor activity (LA) in freely moving mice, using a commercially available telemetry and data acquisition system. The system comprises a new, small radio-telemetry transmitter implanted in the peritoneal cavity, a receiver board placed underneath the home cage, a multiplexer and a computer-based data acquisition system. The signals from the receiver were consolidated by the multiplexer and were stored and analysed by the computer. The telemetered pressure signals (absolute pressure) were corrected automatically for changes in atmospheric pressure measured by an ambient pressure monitor. The effects of implantation on animal behaviour, and, after the animals had recovered, the effects of handling on the SP, DP, MAP and HR were examined. The radio-telemetry system for recording the SP, DP, MAP and HR provides an accurate and reliable method for monitoring the direct effects of handling on SP, DP, MAP and HR. In addition, by using this new blood pressure (BP) transmitter, we maintain that BP measurements in freely moving mice are more efficient, reliable, and less labour-intensive than the measurement techniques described in the literature thus far.
本文首次描述了使用市售遥测和数据采集系统，在自由活动的小鼠中记录收缩压（SP）、舒张压（DP）、平均动脉压（MAP）以及心率（HR）和运动活动（LA）的可能性。该系统包括一个植入腹腔的新型小型无线电遥测发射器、一个放置在饲养笼下方的接收板、一个多路复用器和一个基于计算机的数据采集系统。来自接收器的信号由多路复用器整合，并由计算机进行存储和分析。遥测压力信号（绝对压力）会根据环境压力监测器测量的大气压力变化自动校正。研究了植入对动物行为的影响，以及动物恢复后处理对SP、DP、MAP和HR的影响。用于记录SP、DP、MAP和HR的无线电遥测系统为监测处理对SP、DP、MAP和HR的直接影响提供了一种准确可靠的方法。此外，通过使用这种新型血压（BP）发射器，我们认为在自由活动的小鼠中进行血压测量比迄今为止文献中描述的测量技术更高效、可靠且劳动强度更低。
