Stephens P J, Thomson A J, Keiderling T A, Rawlings J, Rao K K, Hall D O
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5273-5. doi: 10.1073/pnas.75.11.5273.
We report magnetic circular dichroism (MCD) spectra of 4-Fe iron-sulfur clusters in the iron-sulfur proteins Chromatium high-potential iron protein (HIPIP), Bacillus stearothermophilus ferredoxin and Clostridium pasteurianum ferredoxin. The MCD is found to vary significantly with cluster oxidation state but is relatively insensitive to the nature of the protein. The spectra obtained are compared with the corresponding spectra of iron-sulfur proteins containing 2-Fe clusters. It is concluded that MCD is useful for the characterization of iron-sulfur cluster type and oxidation state in iron-sulfur proteins and is superior for this purpose to absorption and natural circular dichroism spectroscopy.
我们报告了铁硫蛋白中的4-铁铁硫簇的磁圆二色性（MCD）光谱，这些铁硫蛋白包括嗜铬高温铁蛋白（HIPIP）、嗜热脂肪芽孢杆菌铁氧还蛋白和巴氏梭菌铁氧还蛋白。发现MCD随簇的氧化态有显著变化，但对蛋白质的性质相对不敏感。将获得的光谱与含有2-铁簇的铁硫蛋白的相应光谱进行了比较。得出的结论是，MCD可用于表征铁硫蛋白中铁硫簇的类型和氧化态，并且在这方面优于吸收光谱和自然圆二色光谱。
