Thomson A J, Cammack R, Hall D O, Rao K K, Briat B, Rivoal J C, Badoz J
Biochim Biophys Acta. 1977 Jul 22;493(1):132-41. doi: 10.1016/0005-2795(77)90266-5.
Variable temperature magnetic circular dichroism (MCD) spectra of a number of two-iron ferredoxins have been measured. The spectra of fully oxidised spinach and Spirulina maxima ferredoxin are independent of temperature between room temperature and 18 K, showing that no contribution to the room temperature MCD spectrum arises from the small population of low-lying excited states originating from the exchange coupling. However, the low temperature MCD spectra of the half-reduced proteins spinach and Spirulina maxima ferredoxin and adrenodoxin are all reasonably intense and temperature dependent. An interpretation of the spectrum of the charge-transfer region is suggested by starting with the assignments previously obtained from rubredoxin.
已测量了多种双铁铁氧化还原蛋白的变温磁圆二色性（MCD）光谱。在室温至18 K之间，完全氧化的菠菜和极大螺旋藻铁氧化还原蛋白的光谱与温度无关，这表明室温MCD光谱并非源于由交换耦合产生的少量低能激发态。然而，半还原蛋白菠菜和极大螺旋藻铁氧化还原蛋白以及肾上腺铁氧化还原蛋白的低温MCD光谱都相当强烈且依赖于温度。通过从先前从红氧还蛋白获得的归属开始，对电荷转移区域的光谱进行了解释。
