Kallies René, Kiesel Bärbel, Schmidt Matthias, Kacza Johannes, Ghanem Nawras, Narr Anja, Zopfi Jakob, Wick Lukas Y, Hackermüller Jörg, Harms Hauke, Chatzinotas Antonis
Department of Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ, 04318 Leipzig, Germany.
Department of Isotope Biogeochemistry, ProVis - Centre for Chemical Microscopy, Helmholtz Centre for Environmental Research - UFZ, 04318 Leipzig, Germany.
Stand Genomic Sci. 2017 Feb 2;12:20. doi: 10.1186/s40793-017-0235-5. eCollection 2017.
vB_PspS-H40/1 is a lytic phage that infects sp. strain H40. Both, the phage and its host were isolated in the 1970s from seawater samples collected from the North Sea near the island of Helgoland, Germany. The phage particle has an icosahedral capsid with a diameter of ~43 to 45 nm and a long non-contractile tail of ~68 nm in length, a typical morphology for members of the family. The linear dsDNA genome of phage vB_PspS-H40/1 has a sequence length of 45,306 bp and a GC content of 40.6%. The genome has a modular structure and contains a high proportion of sequence information for hypothetical proteins, typically seen in phage genome sequences. This is the first report of the complete genome sequence of this lytic phage, which has been frequently used since the 1990s as biological tracer in hydrogeological transport studies.
vB_PspS-H40/1是一种裂解性噬菌体，可感染 sp. 菌株H40。该噬菌体及其宿主均于20世纪70年代从德国黑尔戈兰岛附近北海采集的海水样本中分离得到。噬菌体颗粒具有直径约为43至45纳米的二十面体衣壳和约68纳米长的非收缩性长尾，这是该家族成员的典型形态。噬菌体vB_PspS-H40/1的线性双链DNA基因组序列长度为45,306碱基对，GC含量为40.6%。该基因组具有模块化结构，并且包含高比例的假设蛋白质序列信息，这在噬菌体基因组序列中很常见。这是关于这种裂解性噬菌体完整基因组序列的首次报告，自20世纪90年代以来，它经常被用作水文地质传输研究中的生物示踪剂。
