Gong Zheng, Wang Min, Yang Qingwei, Li Zhongshi, Xia Jun, Gao Yu, Jiang Yong, Meng Xue, Liu Zhaoyang, Yang Ding, Zhang Fangfei, Shao Hongbing, Wang Duobing
College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, 266003, China.
Curr Microbiol. 2017 Jul;74(7):832-839. doi: 10.1007/s00284-017-1244-8. Epub 2017 Apr 20.
Phage PH357, a novel lytic Pseudoalteromonas lipolytica phage belonging to the Myoviridae family was isolated from the Yangtze River estuary. The microbiological characterization demonstrated that phage PH357 is stable from -20 to 60 °C and the optimal pH 7. The one-step growth curve showed a latent period of 20 min, a rise period of 20 min, and the average burst size was about 85 virions per cell. Complete genome of phage PH357 was determined. Genome of phage PH357 consisted of a linear, double-stranded 136,203 bp DNA molecule with 34.58% G + C content, and 242 putative open reading frames (ORFs) without tRNA. All the predicted ORFs were classified into eight functional groups, including DNA replication, regulation and nucleotide metabolism, transcription, translation, phage packaging, phage structure, lysis, host or phage interactions, and hypothetical protein. A phylogenetic analysis showed that phage PH357 had similarity to the previously published Pseudoalteromonas phage PH101 and Vibrio phages. Furthermore, the study of phage PH357 genome will provide useful information for further research on the interaction between phages and their hosts.
噬菌体PH357是一种新型的裂解性解脂假交替单胞菌噬菌体，属于肌尾噬菌体科，从长江口分离得到。微生物学特性表明，噬菌体PH357在-20至60°C之间稳定，最适pH值为7。一步生长曲线显示潜伏期为20分钟，上升期为20分钟，平均裂解量约为每个细胞85个病毒粒子。测定了噬菌体PH357的完整基因组。噬菌体PH357的基因组由一个线性双链136,203 bp的DNA分子组成，G + C含量为34.58%，有242个推定的开放阅读框（ORF），没有tRNA。所有预测的ORF被分为八个功能组，包括DNA复制、调控和核苷酸代谢、转录、翻译、噬菌体包装、噬菌体结构、裂解、宿主或噬菌体相互作用以及假定蛋白。系统发育分析表明，噬菌体PH357与先前发表的假交替单胞菌噬菌体PH101和弧菌噬菌体相似。此外，对噬菌体PH357基因组的研究将为进一步研究噬菌体与其宿主之间的相互作用提供有用信息。
