Groveman Bradley R, Orrú Christina D, Hughson Andrew G, Bongianni Matilde, Fiorini Michele, Imperiale Daniele, Ladogana Anna, Pocchiari Maurizio, Zanusso Gianluigi, Caughey Byron
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute for Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
Department of Neurological and Movement Sciences, University of Verona, Verona, Italy.
Ann Clin Transl Neurol. 2016 Dec 27;4(2):139-144. doi: 10.1002/acn3.378. eCollection 2017 Feb.
Real-Time Quaking-Induced Conversion (RT-QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non-prion disease patients, we provide the first direct and concurrent comparison of our improved RT-QuIC assay to our previous assay, which is similar to those commonly used internationally for CJD diagnosis. This extended comparison demonstrated a ~21% increase in diagnostic sensitivity, a 2-day reduction in average detection time, and 100% specificity.
对人类脑脊液（CSF）进行实时震颤诱导转化（RT-QuIC）检测，在区分散发性克雅氏病（CJD）患者与非朊病毒病患者方面具有高度的敏感性和特异性。在此，我们使用了113例CJD患者和64例非朊病毒病患者的脑脊液样本，首次对改进后的RT-QuIC检测方法与之前的检测方法进行了直接且同步的比较，之前的检测方法与国际上常用于CJD诊断的方法类似。这次扩展比较显示，诊断敏感性提高了约21%，平均检测时间缩短了2天，特异性达到100%。
